Cloned (Comment) | Organism |
---|---|
recombinant expression of the C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)/pET30b-hylp | Streptococcus pyogenes phage H4489A |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | weak inhibition | Streptococcus pyogenes phage H4489A | |
Co2+ | complete inhibition | Streptococcus pyogenes phage H4489A | |
Cu2+ | complete inhibition | Streptococcus pyogenes phage H4489A | |
Ni2+ | complete inhibition | Streptococcus pyogenes phage H4489A | |
SDS | complete inhibition | Streptococcus pyogenes phage H4489A | |
Triton X-100 | weak inhibition | Streptococcus pyogenes phage H4489A | |
Tween 80 | weak inhibition | Streptococcus pyogenes phage H4489A | |
Zn2+ | strong inhibition | Streptococcus pyogenes phage H4489A |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
hyaluronate | pH 5.5, 37°C, recombinant enzyme | Streptococcus pyogenes phage H4489A |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | weak activation | Streptococcus pyogenes phage H4489A |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, about, recombinant His6-tagged enzyme, SDS-PAGE | Streptococcus pyogenes phage H4489A |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronate | Streptococcus pyogenes phage H4489A | efficient degradation of hyaluronate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes phage H4489A | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 53.4fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and cation exchange chromatography | Streptococcus pyogenes phage H4489A |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.62 | - |
pH 5.5, 37°C, purified recombinant enzyme | Streptococcus pyogenes phage H4489A |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronate | efficient degradation of hyaluronate | Streptococcus pyogenes phage H4489A | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, about, recombinant His6-tagged enzyme, SDS-PAGE | Streptococcus pyogenes phage H4489A |
Synonyms | Comment | Organism |
---|---|---|
HA lyase | - |
Streptococcus pyogenes phage H4489A |
hyaluronate lyase | - |
Streptococcus pyogenes phage H4489A |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Streptococcus pyogenes phage H4489A |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | activity range, profile overview | Streptococcus pyogenes phage H4489A |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | purified recombinant enzyme, stable | Streptococcus pyogenes phage H4489A |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Streptococcus pyogenes phage H4489A |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | activity range, profile overview | Streptococcus pyogenes phage H4489A |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | purified recombinant enzyme, stable | Streptococcus pyogenes phage H4489A |