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Literature summary for 4.2.2.1 extracted from
- Identification and characterisation of hyaluronate lyase from Streptococcus suis (2004), Microb. Pathog., 36, 327-335.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene hyl, DNA and amino acid sequence determination and analysis, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptococcus suis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of an enzyme deficient strain by allelic relacement knockout of serotype 7 | Streptococcus suis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54260 | - |
x * 54260, sequence calculation | Streptococcus suis |
| 126500 | 130000 | amino acid sequence calculation and native PAGE | Streptococcus suis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronic acid | Streptococcus suis | the enzyme is essential for growth on hyaluronate as carbon source | hyaluronic acid oligomers | - |
? |  |
| additional information | Streptococcus suis | the enzyme is involved in development of meningitis in infected pigs | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus suis | Q8VLQ8 | hyaluronidase precursor; gene hylAP1/7, serotype 7 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from serotype 7 to over 95% purity, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) | Streptococcus suis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| [hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate | catalytic residues are Asn349, His399, and Tyr408 | Streptococcus suis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hyaluronic acid | the enzyme is essential for growth on hyaluronate as carbon source | Streptococcus suis | hyaluronic acid oligomers | - |
? | |
| hyaluronic acid | native and recombinant serotype 7 enzyme, human umbelical cord hyaluronate | Streptococcus suis | hyaluronic acid oligomers | - |
? | |
| additional information | the enzyme is involved in development of meningitis in infected pigs | Streptococcus suis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54260, sequence calculation | Streptococcus suis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hyaluronidase | - |
Streptococcus suis |
| HylA7 | - |
Streptococcus suis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Streptococcus suis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Streptococcus suis |
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Release 2023.1 (January 2023)
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