A425K |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
A487S |
site-directed mutagenesis, unaltered activity compared to the wild-type enzyme |
Streptococcus agalactiae |
D170A |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
D170E |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
D373H |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
D373H/K466Y/D478Q |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
D432A |
site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
D473E |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
D473G |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
D478Q |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
D494T |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
D494T/K466Y |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
D537A |
site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
E366S |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
E366S/G367R |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
E440V |
site-directed mutagenesis, mutation of a residue in the putative EF hand motif, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
G367R |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
G434A |
site-directed mutagenesis, mutation of a residue in the putative EF hand motif, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
G541A |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
H479A |
site-directed mutagenesis, inactive mutant |
Streptococcus agalactiae |
H479G |
site-directed mutagenesis, inactive mutant |
Streptococcus agalactiae |
I544D |
site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
K437A |
site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
K437A/R542A |
site-directed mutagenesis, mutation of residues in the putative EF hand motif, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
K466Y |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
K466Y/D478Q |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
K466Y/D478Q/K725L/T726W |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
K725L/T726W |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
K861E |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
M659G/D661K |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
M659G/D661K/K725L/T726W |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
N312A |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
N370A |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
N429A |
site-directed mutagenesis, inactive mutant |
Streptococcus agalactiae |
N660A |
site-directed mutagenesis, inactive mutant |
Streptococcus agalactiae |
R321L/R322Q |
site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
R380Q |
site-directed mutagenesis, unaltered activity compared to the wild-type enzyme |
Streptococcus agalactiae |
R380Q/A478S |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
R540A |
site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
R542A |
site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
R546A |
site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
S539A |
site-directed mutagenesis, mutation of a residue in the putative EF hand motif, unaltered activity compared to the wild-type enzyme |
Streptococcus agalactiae |
S545V |
site-directed mutagenesis, mutation of a residue in the putative EF hand motif, slightly reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
W371A |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
W371A/W372A |
site-directed mutagenesis, inactive mutant |
Streptococcus agalactiae |
W372A |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
Y484F |
site-directed mutagenesis, reduced activity compared to the wild-type enzyme |
Streptococcus agalactiae |
Y488F |
site-directed mutagenesis, inactive mutant |
Streptococcus agalactiae |
Y488L |
site-directed mutagenesis, inactive mutant |
Streptococcus agalactiae |
Y488T |
site-directed mutagenesis, inactive mutant |
Streptococcus agalactiae |