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Literature summary for 4.2.2.1 extracted from

  • Pritchard, D.G.; Trent, J.O.; Zhang, P.; Egan, M.L.; Baker, J.R.
    Characterization of the active site of group B streptococcal hyaluronan lyase (2000), Proteins Struct. Funct. Genet., 40, 126-134.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli Streptococcus agalactiae

Protein Variants

Protein Variants Comment Organism
A425K site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
A487S site-directed mutagenesis, unaltered activity compared to the wild-type enzyme Streptococcus agalactiae
D170A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
D170E site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
D373H site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
D373H/K466Y/D478Q site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
D432A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
D473E site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
D473G site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
D478Q site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
D494T site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
D494T/K466Y site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
D537A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
E366S site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
E366S/G367R site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
E440V site-directed mutagenesis, mutation of a residue in the putative EF hand motif, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
G367R site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
G434A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
G541A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
H479A site-directed mutagenesis, inactive mutant Streptococcus agalactiae
H479G site-directed mutagenesis, inactive mutant Streptococcus agalactiae
I544D site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
K437A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
K437A/R542A site-directed mutagenesis, mutation of residues in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
K466Y site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
K466Y/D478Q site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
K466Y/D478Q/K725L/T726W site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
K725L/T726W site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
K861E site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
M659G/D661K site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
M659G/D661K/K725L/T726W site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
N312A site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
N370A site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
N429A site-directed mutagenesis, inactive mutant Streptococcus agalactiae
N660A site-directed mutagenesis, inactive mutant Streptococcus agalactiae
R321L/R322Q site-directed mutagenesis, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
R380Q site-directed mutagenesis, unaltered activity compared to the wild-type enzyme Streptococcus agalactiae
R380Q/A478S site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
R540A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
R542A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
R546A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
S539A site-directed mutagenesis, mutation of a residue in the putative EF hand motif, unaltered activity compared to the wild-type enzyme Streptococcus agalactiae
S545V site-directed mutagenesis, mutation of a residue in the putative EF hand motif, slightly reduced activity compared to the wild-type enzyme Streptococcus agalactiae
W371A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
W371A/W372A site-directed mutagenesis, inactive mutant Streptococcus agalactiae
W372A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
Y484F site-directed mutagenesis, reduced activity compared to the wild-type enzyme Streptococcus agalactiae
Y488F site-directed mutagenesis, inactive mutant Streptococcus agalactiae
Y488L site-directed mutagenesis, inactive mutant Streptococcus agalactiae
Y488T site-directed mutagenesis, inactive mutant Streptococcus agalactiae

Inhibitors

Inhibitors Comment Organism Structure
2,3-Butanedione inactivation, arginine-specific reagent Streptococcus agalactiae
Tetranitromethane inactivation, tyrosine-specific reagent Streptococcus agalactiae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Streptococcus agalactiae

Organism

Organism UniProt Comment Textmining
Streptococcus agalactiae
-
serotype III GBS strain 3502
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli Streptococcus agalactiae

Reaction

Reaction Comment Organism Reaction ID
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate His479 is critical for activity, active site structure, mechanism Streptococcus agalactiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of wild-type and mutant enzymes in diameter of clear zone produced on HA plates in centimeters Streptococcus agalactiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronan
-
Streptococcus agalactiae 2-acetamido-2-deoxy-3-(beta-D-4-deoxy-gluco-4-enepyranosyluronic acid)-glucose + ?
-
?

Subunits

Subunits Comment Organism
More homology model from crystal structure Streptococcus agalactiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Streptococcus agalactiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Streptococcus agalactiae