Literature summary for 4.2.2.1 extracted from

Jedrzejas, M.J.; Mello, L.V.; de Groot, B.L.; Li, S.
Mechanism of hyaluronan degradation by Streptococcus pneumoniae hyaluronate lyase. Structures of complexes with the substrate (2002), J. Biol. Chem., 277, 28287-28297.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of mutant Y408F in Escherichia coli	Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment)	Organism
5 mg/ml Y408 mutant enzyme complexed with tetra- and hexasaccharide hyaluronan substrates, in 10 mM Tris-HCl, pH 7.4, 2 mM EDTA, 1 mM DL-dithiothreitol, against 100 mM sodium cacodylate, pH 6.0, 2.9 M ammonium sulfate, 5 mM EDTA, hanging drop vapour diffusion method, 0.002 ml equal volumes of protein and reservoir solution, 22°C, equilibration against 1 ml reservoir solution, several days, X-ray diffraction structure determination and analysis at 1.52-2.0 A resolution	Streptococcus pneumoniae

Crystallization (Comment)

Organism

5 mg/ml Y408 mutant enzyme complexed with tetra- and hexasaccharide hyaluronan substrates, in 10 mM Tris-HCl, pH 7.4, 2 mM EDTA, 1 mM DL-dithiothreitol, against 100 mM sodium cacodylate, pH 6.0, 2.9 M ammonium sulfate, 5 mM EDTA, hanging drop vapour diffusion method, 0.002 ml equal volumes of protein and reservoir solution, 22°C, equilibration against 1 ml reservoir solution, several days, X-ray diffraction structure determination and analysis at 1.52-2.0 A resolution

Streptococcus pneumoniae

Protein Variants

Protein Variants	Comment	Organism
Y408F	enzyme substrate complex structure determination by crystallization	Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
hyaluronan	Streptococcus pneumoniae	extracellular enzyme facilitates the invasion and spreading of the pathogenic bacterium in the host tissue by degrading the hosts' extracellular matrix component in connective tissue	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate	Asn349, His399, and Tyr408 are involved in catalysis, flexible, allosteric behavior of the enzyme, mechanism, active site structure and substrate binding and interactions, overview	Streptococcus pneumoniae	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activity in different isolated strains from several sources, overview	Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
hyaluronan	extracellular enzyme facilitates the invasion and spreading of the pathogenic bacterium in the host tissue by degrading the hosts' extracellular matrix component in connective tissue	Streptococcus pneumoniae	?	-	?
hyaluronan	-	Streptococcus pneumoniae	3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine	unsaturated disaccharide units	?
hyaluronan hexasaccharide	the longest segment that binds entirely within the active site, a cleft between alpha- and beta-domain	Streptococcus pneumoniae	3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine	unsaturated disaccharide units	?
hyaluronan tetrasaccharide	-	Streptococcus pneumoniae	3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine	unsaturated disaccharide units	?

Synonyms

Synonyms	Comment	Organism
spnHL	-	Streptococcus pneumoniae