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Literature summary for 4.2.2.1 extracted from

  • Hill, J.
    Purification and properties of streptococcal hyaluronate lyase (1976), Infect. Immun., 14, 726-735.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
reducing agents required to stabilize the purified enzyme Streptococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
hyaluronate
-
Streptococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, SDS-PAGE Streptococcus sp.
70000
-
gel filtration Streptococcus sp.

Organism

Organism UniProt Comment Textmining
Streptococcus sp.
-
group A type 4
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification glycoprotein Streptococcus sp.

Purification (Commentary)

Purification (Comment) Organism
-
Streptococcus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Streptococcus sp.

Storage Stability

Storage Stability Organism
-20°C, stable for 2 years Streptococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hyaluronate
-
Streptococcus sp. alpha-4-deoxy-L-threo-hex-4-enopyranosyluronic acid-beta-1,3--N-acetyl-glucosamine
-
?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Streptococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
room temperature, 3 h, 50% loss of activity Streptococcus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Streptococcus sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 6.8 pH 6.0: optimum, pH 6.8: 40% of maximal activity Streptococcus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
30 min, irreversible inactivation Streptococcus sp.
7
-
above pH 7.0, 24 h, irreversible loss of activity Streptococcus sp.
8.6
-
10 min, complete loss of activity Streptococcus sp.