General Stability | Organism |
---|---|
reducing agents required to stabilize the purified enzyme | Streptococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
hyaluronate | - |
Streptococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Streptococcus sp. |
70000 | - |
gel filtration | Streptococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus sp. | - |
group A type 4 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | glycoprotein | Streptococcus sp. |
Purification (Comment) | Organism |
---|---|
- |
Streptococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptococcus sp. |
Storage Stability | Organism |
---|---|
-20°C, stable for 2 years | Streptococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hyaluronate | - |
Streptococcus sp. | alpha-4-deoxy-L-threo-hex-4-enopyranosyluronic acid-beta-1,3--N-acetyl-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Streptococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
room temperature, 3 h, 50% loss of activity | Streptococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Streptococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 6.8 | pH 6.0: optimum, pH 6.8: 40% of maximal activity | Streptococcus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
30 min, irreversible inactivation | Streptococcus sp. |
7 | - |
above pH 7.0, 24 h, irreversible loss of activity | Streptococcus sp. |
8.6 | - |
10 min, complete loss of activity | Streptococcus sp. |