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Literature summary for 4.2.1.B9 extracted from
- Divinyl ether synthesis in garlic bulbs (2008), J. Exp. Bot., 59, 907-915.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli. Expression in Arabidopsis shows that 9-lipoxygenase-derived colnelenic acid dominates 24 h after wounding | Allium sativum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the KM-value for (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate is above 0.1 mM | Allium sativum | |
| 0.01 | - |
13-hydroperoxy-octadecatrienoate | pH 7.0 | Allium sativum |  |
| 0.02 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | pH 7.0 | Allium sativum | |
| 0.03 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | pH 7.0 | Allium sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plastid | - |
Allium sativum | 9536 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52800 | - |
x * 52800, calculated from sequence | Allium sativum |
| 52840 | - |
deduced from amino acid sequence | Allium sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | Allium sativum | the prominent divinyl ether in garlic is the 13-lipoxygenase-derived etheroleic acid. Expression in Arabidopsis shows that 9-lipoxygenase-derived colnelenic acid dominates 24 h after wounding | (9Z,11E)-12-[(1E)-hex-1-en-1-yloxy]dodeca-9,11-dienoic acid + H2O | i.e. etheroleic acid | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Allium sativum | Q2WE96 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Allium sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bulb | transcripts predominant in below-ground organs of garlic. By exogenous application of salicylic acid and sorbitol, but not by methyljasmonate, the transcript is also induced in leaves | Allium sativum | - |
| leaf | hardly detectable in leaves, transcripts are predominant in below-ground organs of garlic. By exogenous application of salicylic acid and sorbitol, but not by methyljasmonate, the transcript is also induced in leaves | Allium sativum | - |
| root | transcripts predominant in below-ground organs of garlic. By exogenous application of salicylic acid and sorbitol, but not by methyljasmonate, the transcript is also induced in leaves | Allium sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | - |
Allium sativum | (8E)-9-[(1E,3Z)-nona-1,3-dien-1-yloxy]non-8-enoate + H2O | i.e. colneleate | ? | |
| (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | - |
Allium sativum | (8Z)-9-[(1E,3Z)-nona-1,3-dien-1-yloxy]non-8-enoate + H2O | i.e. colnelenate | ? | |
| (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | the prominent divinyl ether in garlic is the 13-lipoxygenase-derived etheroleic acid. Expression in Arabidopsis shows that 9-lipoxygenase-derived colnelenic acid dominates 24 h after wounding | Allium sativum | (9Z,11E)-12-[(1E)-hex-1-en-1-yloxy]dodeca-9,11-dienoic acid + H2O | i.e. etheroleic acid | ? | |
| (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | - |
Allium sativum | (9Z,11E)-12-[(1E)-hex-1-en-1-yloxy]dodeca-9,11-dienoate + H2O | i.e. etheroleate | ? | |
| (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate | - |
Allium sativum | (9Z,11E)-12-[(1E,3Z)-hexa-1,3-dien-1-yloxy]dodeca-9,11-dienoate + H2O | i.e. etherolenate | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52800, calculated from sequence | Allium sativum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 13/9-DES | - |
Allium sativum |
| 13/9-divinyl ether synthase | - |
Allium sativum |
| 9/13-DES | - |
Allium sativum |
| 9/13-divinyl ether synthase | - |
Allium sativum |
| etheroleic acid/colneleic acid synthase | - |
Allium sativum |
| More | member of the CYP74D subfamily | Allium sativum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | pH 7.0 | Allium sativum | |
| 40 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | pH 7.0 | Allium sativum | |
| 70 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | pH 7.0 | Allium sativum | |
| 550 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | pH 7.0 | Allium sativum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Allium sativum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | cytochrome P450 | Allium sativum | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Allium sativum | by exogenous application of salicylic acid and sorbitol, but not by methyljasmonate, the transcript is induced in leaves | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 500 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate | pH 7.0 | Allium sativum | |
| 2333 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate | pH 7.0 | Allium sativum | |
| 4000 | - |
13-hydroperoxy-octadecatrienoate | pH 7.0 | Allium sativum | |
| 4400 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate | pH 7.0 | Allium sativum | |
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