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Literature summary for 4.2.1.B28 extracted from

  • Banta, A.B.; Wei, J.H.; Welander, P.V.
    A distinct pathway for tetrahymanol synthesis in bacteria (2015), Proc. Natl. Acad. Sci. USA, 112, 13478-13483.
    View publication on PubMed
Search for more literature for 4.2.1.B28

Cloned(Commentary)

Cloned (Comment) Organism
gene MEALZ_1626 or rhs, sequence comparisons and phylogenetic analysis, phylogenetics and metagenomics, recombinant expression in Methylococcus capsulatus Bath strains Methylotuvimicrobium alcaliphilum
gene Rpal_0860 or ths, sequence comparisons and phylogenetic analysis, phylogenetics and metagenomics, recombinant expression in and functional complementation of DELTAths deletion mutant strain of Methylomicrobium alcaliphilum Rhodopseudomonas palustris

Protein Variants

Protein Variants Comment Organism
additional information construction of a DELTAths deletion mutant, functional complementation by expression of gene Rpal_0860, encoding wild-type Ths, and gene shc from Methylomicrobium alcaliphilum strain 20Z Methylotuvimicrobium alcaliphilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rhodopseudomonas palustris the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene ?
-
 ?
additional information Methylotuvimicrobium alcaliphilum the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene ?
-
 ?
additional information Rhodopseudomonas palustris TIE-1 the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene ?
-
 ?
additional information Methylotuvimicrobium alcaliphilum DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methylotuvimicrobium alcaliphilum G4SZH3
-
-
Methylotuvimicrobium alcaliphilum DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z G4SZH3
-
-
Rhodopseudomonas palustris B3QED8
-
-
Rhodopseudomonas palustris TIE-1 B3QED8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hopene + H2O
-
 Rhodopseudomonas palustris tetrahymanol
-
 ?
hopene + H2O
-
 Rhodopseudomonas palustris TIE-1 tetrahymanol
-
 ?
additional information the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene Rhodopseudomonas palustris ?
-
 ?
additional information the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene Methylotuvimicrobium alcaliphilum ?
-
 ?
additional information the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene Rhodopseudomonas palustris TIE-1 ?
-
 ?
additional information the enzyme catalyzes steps in the synthesis of tetrahymanol from hopene Methylotuvimicrobium alcaliphilum DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z ?
-
 ?

Synonyms

Synonyms Comment Organism
MEALZ_1626
-
 Methylotuvimicrobium alcaliphilum
Rpal_0860
-
 Rhodopseudomonas palustris
Ths
-
 Rhodopseudomonas palustris
Ths
-
 Methylotuvimicrobium alcaliphilum

General Information

General Information Comment Organism
evolution tetrahymanol synthase (Ths) is found in a variety of bacterial genomes, including aerobic methanotrophs, nitrite-oxidizers, and sulfate-reducers, and in a subset of aquatic and terrestrial metagenomes. The potential to produce tetrahymanol is more widespread in the bacterial domain than previously thought. Ths is not encoded in any eukaryotic genomes, nor is it homologous to eukaryotic squalene-tetrahymanol cyclase (EC 4.2.1.123), which catalyzes the cyclization of squalene directly to tetrahymanol Rhodopseudomonas palustris
evolution tetrahymanol synthase (Ths) is found in a variety of bacterial genomes, including aerobic methanotrophs, nitrite-oxidizers, and sulfate-reducers, and in a subset of aquatic and terrestrial metagenomes. The potential to produce tetrahymanol is more widespread in the bacterial domain than previously thought. Ths is not encoded in any eukaryotic genomes, nor is it homologous to eukaryotic squalene-tetrahymanol cyclase (EC 4.2.1.123), which catalyzes the cyclization of squalene directly to tetrahymanol Methylotuvimicrobium alcaliphilum
additional information hopanoids, tetrahymanol, and sterol structures observed in Methylomicrobium alcaliphilum strain 20Z, overview Methylotuvimicrobium alcaliphilum
physiological function tetrahymanol, whose catalysis is synthesized by tetrahymanol synthase, is a polycyclic triterpenoid lipid. Enzyme tetrahymanol synthase (Ths) functions on hopenes rather than squalene, it first demethylates squalene to form C23-norsqualene, which is then converted to a demethyl derivative of tetrahymanol by squalene-hopene cyclase (Shc, EC 5.4.99.17). This pathway requires the addition of a methyl group at C-23 to form tetrahymanol. Enzyme Shc cyclizes squalene to diploptene, and Ths then expands the E ring to form tetrahymanol, overview Rhodopseudomonas palustris
physiological function tetrahymanol, whose catalysis is synthesized by tetrahymanol synthase, is a polycyclic triterpenoid lipid. Enzyme tetrahymanol synthase (Ths) functions on hopenes rather than squalene, it first demethylates squalene to form C23-norsqualene, which is then converted to a demethyl derivative of tetrahymanol by squalene-hopene cyclase (Shc, EC 5.4.99.17). This pathway requires the addition of a methyl group at C-23 to form tetrahymanol. Enzyme Shc cyclizes squalene to diploptene, and Ths then expands the E ring to form tetrahymanol, overview Methylotuvimicrobium alcaliphilum