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Literature summary for 4.2.1.B20 extracted from

  • Ke, D.; Caiyin, Q.; Zhao, F.; Liu, T.; Lu, W.
    Heterologous biosynthesis of triterpenoid ambrein in engineered Escherichia coli (2018), Biotechnol. Lett., 40, 399-404 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of onoceroid synthase BmeTC from Bacillus megaterium with Saccharomyces cerevisiae squalene synthase (gene ScERG9) and Alicyclobacillus acidocaldarius squalene-hopene synthase mutant D377C (mutated gene shc) in Escherichia coli strain MEP43 (with deleted the beta-carotene synthesis module) under control of constitutive J23119 promoter, subcloning in Escherichia coli strain DH5alpha Priestia megaterium

Protein Variants

Protein Variants Comment Organism
additional information establishing of an (+)-ambrein production system by coexpressing the onoceroid synthase BmeTC from Bacillus megaterium with Saccharomyces cerevisiae squalene synthase (gene ScERG9) and Alicyclobacillus acidocaldarius squalene-hopene synthase mutant D377C (mutated gene shc) in Escherichia coli strain MEP43 (with deleted the beta-carotene synthesis module). The squalene synthase produces squalene, SHC enzyme mutant D377C produces 3-deoxyachilleol A from squalene, and BmTC forms ambrein from it, method optimization. Optimal temperature for ambrein production is 30-34°C Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-deoxyachilleol A Priestia megaterium
-
(+)-ambrein
-
?
8alpha-hydroxypolypoda-13,17,21-triene Priestia megaterium
-
onoceranoxide + 14beta-hydroxyonocera-8(26)-ene
-
?
squalene Priestia megaterium
-
8alpha-hydroxypolypoda-13,17,21-triene
-
?
tetraprenyl-beta-curcumene + H2O Priestia megaterium
-
baciterpenol A
-
?

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-deoxyachilleol A
-
Priestia megaterium (+)-ambrein
-
?
8alpha-hydroxypolypoda-13,17,21-triene
-
Priestia megaterium onoceranoxide + 14beta-hydroxyonocera-8(26)-ene
-
?
squalene
-
Priestia megaterium 8alpha-hydroxypolypoda-13,17,21-triene
-
?
tetraprenyl-beta-curcumene + H2O
-
Priestia megaterium baciterpenol A
-
?

Synonyms

Synonyms Comment Organism
BmeTC
-
Priestia megaterium
tetraprenyl-beta-curcumene cyclase
-
Priestia megaterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
in vivo assay at Priestia megaterium