Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | 0.1%, stimulates | Eubacterium sp. | |
SDS | 0.05%, about 7fold stimulation | Eubacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
16-Ketoprogesterone | competitive with respect to 16alpha-hydroxypregnenolone and 16alpha-hydroxyprogesterone | Eubacterium sp. | |
16alpha-hydroxypregnenolone | substrate inhibition at high concentrations | Eubacterium sp. | |
cetyltrimethylammonium bromide | no inhibition by NaBr | Eubacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
16alpha-hydroxypregnenolone | - |
Eubacterium sp. | |
0.52 | - |
16alpha-hydroxyprogesterone | - |
Eubacterium sp. | |
0.52 | - |
16alpha-hydroxyprogesterone | pH 6.8 | Eubacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42400 | - |
4 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE | Eubacterium sp. |
42400 | - |
8 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE | Eubacterium sp. |
42400 | - |
x * 42400, SDS-PAGE | Eubacterium sp. |
181000 | - |
tetrameric form, gel filtration | Eubacterium sp. |
181000 | - |
active in two molecular weight forms, gel filtration | Eubacterium sp. |
326000 | - |
octameric form, gel filtration | Eubacterium sp. |
326000 | - |
active in two molecular weight forms, gel filtration | Eubacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eubacterium sp. | - |
- |
- |
Eubacterium sp. 144 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Eubacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | - |
- |
Eubacterium sp. |
0.7094 | - |
- |
Eubacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
16alpha-Hydroxypregnenolone | - |
Eubacterium sp. | ? | - |
? | |
16alpha-Hydroxypregnenolone | - |
Eubacterium sp. 144 | ? | - |
? | |
16alpha-hydroxypregnenolone | - |
Eubacterium sp. | 16-dehydropregnenolone + H2O | - |
? | |
16alpha-hydroxypregnenolone | - |
Eubacterium sp. 144 | 16-dehydropregnenolone + H2O | - |
? | |
16alpha-Hydroxyprogesterone | - |
Eubacterium sp. | 16-Dehydroprogesterone | - |
? | |
16alpha-Hydroxyprogesterone | - |
Eubacterium sp. 144 | 16-Dehydroprogesterone | - |
? | |
16alpha-hydroxyprogesterone | - |
Eubacterium sp. | 16dehydroprogesterone + H2O | - |
? | |
16alpha-hydroxyprogesterone | - |
Eubacterium sp. 144 | 16dehydroprogesterone + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42400, SDS-PAGE | Eubacterium sp. |
octamer | 8 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE | Eubacterium sp. |
tetramer | 4 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE | Eubacterium sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
16-Ketoprogesterone | pH 6.8, reaction with 16alpha-hydroxyprogesterone | Eubacterium sp. | |
0.18 | - |
16-Ketoprogesterone | pH 6.8, reaction with 16alpha-hydroxypregnenolone | Eubacterium sp. |