Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as GST-tagged enzyme | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
purified recombinnat detagged mutant T51S enzyme, 13 mg/ml protein from 0.1 M HEPES-Na, pH 7.5, 2% v/v PEG 400, and 2.0 M ammonium sulfate, X-ray diffraction structure determination and analysis at 1.8 A resolution | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
T51S | the point mutation at the enzyme tetramer interface overcomes the dissociation barrier of the homotetramer and increases the interaction with HNF-1alpha. Presence of an ordered water molecule at the tetramer interface, which may destabilize the homotetramer | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, tag cleavage by thrombin, and p-aminobenzamidine affinity chromatography | Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
DCoH2 unfolding through guanidine is reversible, kinetics, equilibrium unfolding data fit to a two-state model with no apparent intermediate, but folding intermediates are detectable. Proposal of an unfolding pathway in which the tetramer unfolds slowly, but the dimer folds reversibly | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
PCD | - |
Rattus norvegicus |
Pterin-4a-carbinolamine dehydratase | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
metabolism | the PCD activity of the homoteterameric enzyme is involved in the aromatic amino acid metabolism | Rattus norvegicus |
physiological function | the bifunctional protein shows two disparate functions, i.e. dimerization cofactor of HNF-1, DCoH1, and pterin-4a-carbinolamine dehydratase, PCD, that are associated with a change in oligomeric state between dimer and tetramer, overview. The PCD activity of homotetramers aids in aromatic amino acidmetabolism | Rattus norvegicus |