Literature summary for 4.2.1.96 extracted from

Cameron, S.; Fyffe, S.A.; Goldie, S.; Hunter, W.N.
Crystal structures of Toxoplasma gondii pterin-4a-carbinolamine dehydratase and comparisons with mammalian and parasite orthologues (2008), Mol. Biochem. Parasitol., 158, 131-138.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21 (DE3). There is a high degree of structural similarity between Toxoplasma gondii PCD and the mammalian orthologue, Rattus norvegicus PCD, which is identical to the human enzyme	Toxoplasma gondii

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion, 2V6S: 1.4 M ammonium sulfate, 1 M lithium sulfate, 2 mM 7,8-dihydrobiopterin, pH 7.6, space group P 43 21 2, resolution 2.22 A, complex with substrate-like ligand 7,8-dihydrobiopterin TgPCD:BH2 complex 2V6T: 0.4 M potassium-sodium tartrate, pH 7.6, space group P 43 21 2, resolution 3.1 A, apo-enzyme 2V6U: 1.4 M ammonium sulfate, 1 M lithium sulfate, pH 7.6, space group P 43 21 2, resolution 1.6 A	Toxoplasma gondii

Crystallization (Comment)

Organism

sitting drop vapour diffusion, 2V6S: 1.4 M ammonium sulfate, 1 M lithium sulfate, 2 mM 7,8-dihydrobiopterin, pH 7.6, space group P 43 21 2, resolution 2.22 A, complex with substrate-like ligand 7,8-dihydrobiopterin TgPCD:BH2 complex 2V6T: 0.4 M potassium-sodium tartrate, pH 7.6, space group P 43 21 2, resolution 3.1 A, apo-enzyme 2V6U: 1.4 M ammonium sulfate, 1 M lithium sulfate, pH 7.6, space group P 43 21 2, resolution 1.6 A

Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24200	-	asymmetric unit contains two subunits 2 * 24200	Toxoplasma gondii
46000	-	gel filtration	Toxoplasma gondii

Organism

Organism	UniProt	Comment	Textmining
Toxoplasma gondii	Q2Q449	-	-

Purification (Commentary)

Purification (Comment)	Organism
by affinity chromatography over a HisTrap nickel chelating column, followed by TEV cleavage/dialysis at 4°C overnight and then further passage over the a HisTrap nickel column to remove the tag, contaminants and the His-tagged TEV protease. A final purification step using a Superdex 75 size-exclusion column is performed	Toxoplasma gondii

Subunits

Subunits	Comment	Organism
tetramer	asymmetric unit contains two subunits 2 * 24200	Toxoplasma gondii

Synonyms

Synonyms	Comment	Organism
PCD	-	Toxoplasma gondii
Pterin-4a-carbinolamine dehydratase	-	Toxoplasma gondii