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Literature summary for 4.2.1.96 extracted from

  • Hevel, J.M.; Pande, P.; Viera-Oveson, S.; Sudweeks, T.J.; Jaffree, L.S.; Hansen, C.M.; Ayling, J.E.
    Determinants of oligomerization of the bifunctional protein DCoHalpha and the effect on its enzymatic and transcriptional coactivator activities (2008), Arch. Biochem. Biophys., 477, 356-362.
    View publication on PubMed
Search for more literature for 4.2.1.96

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3)pLysS Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information 3 residues, Asn61, Gln45, and Lys98 in DCoHalpha play a role in oligomeric flexibility, which enables DCoHalpha to more readily interact with HNF1alpha and increase DNA binding Homo sapiens
N61D The decreased ability of the N61D mutant to affect HNF1alpha-dependent DNA binding is likely a direct result of altered quaternary structure. Homo sapiens
N61D/Q45R/K98Q site-directed mutagenesis, triple DCoHa mutant (Q45R/K98Q/N61D) is unable to affect HNF1alpha-dependent DNA binding in vitro. Homo sapiens
Q45R/K98Q mutant Q45R/K98Q is not able to affect HNF1alpha-dependent DNA binding in vitro Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin wild-type Homo sapiens
0.0044
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin Q45R/K98Q Homo sapiens
0.0052
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin N61D Homo sapiens
0.0053
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin N61D/Q45R/K98Q Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H0N5
-
-

Purification (Commentary)

Purification (Comment) Organism
at 4°C Homo sapiens

Storage Stability

Storage Stability Organism
mutant proteins require the inclusion of 10% glycerol in the storage buffer to maintain solubility Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin
-
 Homo sapiens (6R)-6-(L-erythro-1,2-dihydroxypropyl)-7,8-dihydro-6H-pterin + H2O
-
 ?
additional information DCoHalpha is coactivator of HNF1alpha-depndent transcription Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer tetramer-dimer equilibrium at lower concentration, wild-type Homo sapiens
tetramer high concentration, wild-type Homo sapiens
tetramer mutant enzyme N61D/Q45R/K98Q shows no evidence of a dimeric spiecies Homo sapiens
tetramer mutant enzyme Q45R/K98Q shows no evidence of a dimeric spiecies Homo sapiens

Synonyms

Synonyms Comment Organism
4a-Hydroxytetrahydrobiopterin dehydratase
-
 Homo sapiens
DCoHalpha
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin N61D Homo sapiens
23
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin N61D/Q45R/K98Q Homo sapiens
23
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin Q45R/K98Q Homo sapiens
24
-
 (6R)-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydro-4a-hydroxypterin wild-type Homo sapiens