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Literature summary for 4.2.1.94 extracted from
- Comparison of scytalone dehydratase activities between scytalone dehydratase inhibitor-sensitive and inhibitor-resistant Magnaporthe oryzae isolates (2017), J. Plant Dis. Protect., 124, 525-532 .
No PubMed abstract available
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | fungal isolates resistant to the melanin biosynthesis inhibitors-dehydratase (MBI-D), which inhibits scytalone dehydratase, are detected in Japan. The isolates have a single point mutation in the gene encoding scytalone dehydratase. In fitness tests, resistant isolates show reduced fitness, compared with that of sensitive isolates. Enzyme kinetics analyses of the scytalone dehydratases from MBI-D-sensitive and MBI-D-resistant isolates reveal that the activity of MBI-D-sensitive-type enzymes is approximately double that of MBI-D-resistant-type enzymes. The sensitive-type enzyme has higher scytalone dehydration activity than that of resistant-type enzyme at temperatures ranging from 15-35°C and at pHs ranging from pH 6.0-9.0. Acquisition of resistance to MBI-D negatively affects the pathogenicity of Magnaporthe oryzae due to decreased scytalone dehydratase activity | Pyricularia oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diclocymet | MBI-D, inhibits scytalone dehydratase | Pyricularia oryzae |  |
| additional information | comparison of scytalone dehydratase activities between scytalone dehydratase inhibitor-sensitive and inhibitor-resistant Magnaporthe oryzae isolates, overview | Pyricularia oryzae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0443 | - |
Scytalone | pH 8.0, 25°C, inhibitor-resistant enzyme | Pyricularia oryzae | |
| 0.0532 | - |
Scytalone | pH 8.0, 25°C, inhibitor-sensitive enzyme | Pyricularia oryzae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| scytalone | Pyricularia oryzae | - |
1,3,8-trihydroxynaphthalene + H2O | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyricularia oryzae | - |
several different MBI-D inhibitor-sensitive and inhibitor-resistant isolates, overview | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from mycelia by ammonium sulfate fractionation, anion exchange chromatography, desalting gel filtration, and another step of different anion exchange chromatography | Pyricularia oryzae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Pyricularia oryzae | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | comparison of scytalone dehydratase activities between scytalone dehydratase inhibitor-sensitive and inhibitor-resistant Magnaporthe oryzae isolates overview | Pyricularia oryzae | ? | - |
? | |
| scytalone | - |
Pyricularia oryzae | 1,3,8-trihydroxynaphthalene + H2O | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
MBI-D-resistant and MBI-D-sensitive isolates | Pyricularia oryzae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 35 | proportions of scytalone catalyzed by the enzymes from the MBI-D-sensitive and MBI-D-resistant isolates are 42.3 and 17.0%, respectively, at 15°C and 52.3 and 23.9%, respectively, at 20°C and 66.6 and 35.6%, respectively, at 25°C and 79.9 and 52.0%, respectively, at 30°C and 84.3 and 60.5%, respectively, at 35°C | Pyricularia oryzae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.6 | - |
Scytalone | pH 8.0, 25°C, inhibitor-resistant enzyme | Pyricularia oryzae | |
| 38.2 | - |
Scytalone | pH 8.0, 25°C, inhibitor-sensitive enzyme | Pyricularia oryzae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
enzyme from MBI-D-sensitive strain | Pyricularia oryzae |
| 8 | - |
enzyme from MBI-D-resistant strain | Pyricularia oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | proportions of scytalone catalyzed by the enzymes from the MBI-D-sensitive and MBI-D-resistant isolates are 47.1 and 28.0%, respectively, at pH 6.0, and 69.3 and 40.4%, respectively, at pH 7.0 and 67.1 and 42.2%, respectively, at pH 8.0, and 40.4 and 27.6%, respectively, at pH 9.0 | Pyricularia oryzae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | mutational acquisition of resistance to MBI-D negatively affects the pathogenicity of Magnaporthe oryzae due to decreased scytalone dehydratase activity | Pyricularia oryzae |
| metabolism | the enzyme is involved in melanin biosynthesis | Pyricularia oryzae |
| additional information | comparison of infection rates of inhibitor MBI-D-sensitive and MBI-D-resistant isolates | Pyricularia oryzae |
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