Cloned (Comment) | Organism |
---|---|
overexpression of V75M in Escherichia coli with or without an N-terminal histidine-tag | Pyricularia oryzae |
Protein Variants | Comment | Organism |
---|---|---|
V75M | inhibition of the mutant enzyme by (1RS,3RS)-2,2-dichloro-N-[(R)-1-(4-chlorophenyl)ethyl]-1-ethyl-3-methylcyclopropanecarboxamide is more than 200fold reduced in comparison with that of the wild-type, about 2fold reduced turnover number for scytalone as compared to wild-type enzyme | Pyricularia oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(1RS,3RS)-2,2-dichloro-N-[(R)-1-(4-chlorophenyl)ethyl]-1-ethyl-3-methylcyclopropanecarboxamide | i.e. carpropamid | Pyricularia oryzae | |
KTU3615A | competitive | Pyricularia oryzae | |
KTU3615B | competitive | Pyricularia oryzae | |
KTU3616A | competitive | Pyricularia oryzae | |
KTU3616B | competitive | Pyricularia oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
Scytalone | mutant enzyme V75M with His-tag | Pyricularia oryzae | |
0.022 | - |
Scytalone | wild-type enzyme | Pyricularia oryzae | |
0.023 | - |
Scytalone | mutant enzyme V75M without His-tag | Pyricularia oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
x * 21000, calculation from nucleotide sequence | Pyricularia oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyricularia oryzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyricularia oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
scytalone | - |
Pyricularia oryzae | 1,3,8-trihydroxynaphthalene + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21000, calculation from nucleotide sequence | Pyricularia oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | Scytalone | mutant enzyme V75M with His-tag | Pyricularia oryzae | |
41 | - |
Scytalone | mutant enzyme V75M without His-tag | Pyricularia oryzae | |
70 | - |
Scytalone | wild-type enzyme | Pyricularia oryzae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000001 | - |
KTU3616B | pH 7.0, 30°C, wild-type enzme | Pyricularia oryzae | |
0.000011 | - |
KTU3616A | pH 7.0, 30°C, wild-type enzme | Pyricularia oryzae | |
0.000024 | - |
KTU3615A | pH 7.0, 30°C, wild-type enzme | Pyricularia oryzae |