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Literature summary for 4.2.1.93 extracted from

  • Niehaus, T.D.; Richardson, L.G.; Gidda, S.K.; Elbadawi-Sidhu, M.; Meissen, J.K.; Mullen, R.T.; Fiehn, O.; Hanson, A.D.
    Plants utilize a highly conserved system for repair of NADH and NADPH hydrates (2014), Plant Physiol., 165, 52-61.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.93

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression as GFP-tagged enzyme in Nicotiana tabacum suspension cells, subcellular localization comprises cytosol, mitochondria, and chloroplasts Arabidopsis thaliana
recombinant expression as GFP-tagged enzyme in Nicotiana tabacum suspension cells, subcellular localization comprises cytosol, mitochondria, and chloroplasts Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00069
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Arabidopsis thaliana
0.00086
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Zea mays
0.00089
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Zea mays
0.00115
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast import assays with purified pea (Pisum sativum) chloroplasts and mitochondria Arabidopsis thaliana 9507
-
chloroplast import assays with purified pea (Pisum sativum) chloroplasts and mitochondria Zea mays 9507
-
cytosol
-
 Arabidopsis thaliana 5829
-
cytosol
-
 Zea mays 5829
-
mitochondrion import assays with purified pea (Pisum sativum) chloroplasts and mitochondria Arabidopsis thaliana 5739
-
mitochondrion import assays with purified pea (Pisum sativum) chloroplasts and mitochondria Zea mays 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Zea mays stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana Col-0 stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana Columbia-0 stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADPH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Zea mays stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADPH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana Col-0 stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADPH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana Columbia-0 stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q94AF2 gene At5g19150
-
Arabidopsis thaliana Col-0 Q94AF2 gene At5g19150
-
Zea mays A0A3L6F337
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide stereospecific for (S)-NAD(P)HX epimer Zea mays ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana Col-0 ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana Columbia-0 ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana ADP + phosphate + NADPH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate stereospecific for (S)-NAD(P)HX epimer Zea mays ADP + phosphate + NADPH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana Col-0 ADP + phosphate + NADPH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana Columbia-0 ADP + phosphate + NADPH
-
 ?

Synonyms

Synonyms Comment Organism
(S)-NAD(P)HX dehydratase
-
 Arabidopsis thaliana
(S)-NAD(P)HX dehydratase
-
 Zea mays

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Arabidopsis thaliana
22
-
 assay at Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.26
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Arabidopsis thaliana
0.3
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Zea mays
0.34
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Arabidopsis thaliana
0.38
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Arabidopsis thaliana
8
-
 assay at Zea mays

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Arabidopsis thaliana
ATP dependent on Zea mays

General Information

General Information Comment Organism
evolution domain architectures of the NAD(P)HX dehydratase enzymes of prokaryotes, yeast, andmammals, and of dehydratase homologue in plants Arabidopsis thaliana
evolution domain architectures of the NAD(P)HX dehydratase enzymes of prokaryotes, yeast, andmammals, and of dehydratase homologue in plants Zea mays
physiological function the enzyme is part of a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites Arabidopsis thaliana
physiological function the enzyme is part of a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites Zea mays

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
262
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Arabidopsis thaliana
358
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Zea mays
509
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Arabidopsis thaliana
524
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Zea mays