Literature summary for 4.2.1.92 extracted from

Brash, A.R.; Niraula, N.P.; Boeglin, W.E.; Mashhadi, Z.
An ancient relative of cyclooxygenase in cyanobacteria is a linoleate 10S-dioxygenase that works in tandem with a catalase-related protein with specific 10S-hydroperoxide lyase activity (2014), J. Biol. Chem., 289, 13101-13111.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene Npun_R5468, expression of the enzyme with N- or C-terminal His6 tags in Escherichia coli strain BL21(DE3)	Nostoc punctiforme

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	x * 45000, about, sequence calculation	Nostoc punctiforme

Organism

Organism	UniProt	Comment	Textmining
Nostoc punctiforme	B2J5P3	gene Npun_R5468	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N- or C-terminal His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis	Nostoc punctiforme

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(8E,10S,12Z)-10-hydroperoxyoctadeca-8,12-dienoic acid	the catalase-related hemoprotein reacts rapidly and specifically with linoleate 10S-hydroperoxide with a hydroperoxide lyase activity specific for the 10S-hydroperoxy enantiomer	Nostoc punctiforme	(8E)-10-oxodecenoic acid + 1-octen-3-ol + (2Z)-octen-1-ol	in a 3:1 ratio, strict enzymatic control in formation of the 3R alcohol configuration with 99% enantiomeric excess	?
additional information	the enzyme mainly acts as an oleate or linoleate dioxygenase, albeit with alpha-linolenic acid very competitive at low substrate concentrations, substrate specificity and kinetics, overview	Nostoc punctiforme	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 45000, about, sequence calculation	Nostoc punctiforme

Synonyms

Synonyms	Comment	Organism
10S-hydroperoxide lyase	-	Nostoc punctiforme
Npun_R5469	-	Nostoc punctiforme

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Release 2023.1 (January 2023)