Literature summary for 4.2.1.92 extracted from

Bandara, P.K.; Takahashi, K.; Sato, M.; Matsuura, H.; Nabeta, K.
Cloning and functional analysis of an allene oxide synthase in Physcomitrella patens (2009), Biosci. Biotechnol. Biochem., 73, 2356-2359.

Search for more literature for 4.2.1.92

Cloned(Commentary)

Cloned (Comment)	Organism
AOS2 cloned and overexpressed in Escherichia coli	Physcomitrium patens
whole ORF of AOS1 amplified and ligated into cloning vector pBluescript SK II(+), sequenced and ligated int vector pET23a and overexpressed in Escherichia coli BL21	Physcomitrium patens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0593	-	(13S)-hydroperoxy-(9Z,11Z,15Z)-octadecatrienoic acid	with 0.0001 mg purified AOS1, in 1 mL of buffer containing 50 mM sodium phosphate, pH 6.0	Physcomitrium patens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
53500	-	sequence analysis	Physcomitrium patens

Organism

Organism	UniProt	Comment	Textmining
Physcomitrium patens	A9S014	-	-
Physcomitrium patens	A9SNA2	-	-

Purification (Commentary)

Purification (Comment)	Organism
AOS1 purified on Ni-NTA resin	Physcomitrium patens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(13S)-hydroperoxy-(9Z,11Z,15Z)-octadecatrienoic acid	by AOS1	Physcomitrium patens	(12,13S)-epoxy-(9Z,11Z,15Z)-octadecatrienoic acid + H2O	-	?

Synonyms

Synonyms	Comment	Organism
allene oxide synthase	-	Physcomitrium patens
AOS1	-	Physcomitrium patens
AOS2	-	Physcomitrium patens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	AOS1	Physcomitrium patens

Expression

Organism	Comment	Expression
Physcomitrium patens	expression of AOS1 is higher than that of AOS2	up

General Information

General Information	Comment	Organism
metabolism	AOS is involved in jasmonic acid biosynthesis. AOS1 operates acid biosynthesis predominantly	Physcomitrium patens
physiological function	expressed AOS2 protein is not obtained in soluble form	Physcomitrium patens

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Release 2023.1 (January 2023)