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Literature summary for 4.2.1.90 extracted from

  • Koivistoinen, O.M.; Arvas, M.; Headman, J.R.; Andberg, M.; Penttilae, M.; Jeffries, T.W.; Richard, P.
    Characterisation of the gene cluster for L-rhamnose catabolism in the yeast Scheffersomyces (Pichia) stipitis (2012), Gene, 492, 177-185.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
the genes for L-rhamnose catabolism RHA1, LRA2, LRA3 and LRA4 but not LADH are clustered, genetic organization, phylogenetic analysis, overview. Functional expression of gene LRA3 in Saccharomyces cerevisiae with a strong constitutive promoter. C-terminally his-tagged protein shows no activity and the activity of the N-terminally tagged protein is reduced by about 95%, so the enzyme is expressed without tagand not purified Scheffersomyces stipitis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics, overview Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-rhamnonate Scheffersomyces stipitis
-
 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis A3LZU6 i.e. Pichia stipitis, gene LRA3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-rhamnonate
-
 Scheffersomyces stipitis 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
Lra3p
-
 Scheffersomyces stipitis

General Information

General Information Comment Organism
metabolism the enzyme is involved in L-rhamnose catabolism. Eukaryotic L-rhamnose pathway, overview Scheffersomyces stipitis