BRENDA - Enzyme Database
show all sequences of 4.2.1.9

Function and maturation of the Fe-S center in dihydroxyacid dehydratase from Arabidopsis

Gao, H.; Azam, T.; Randeniya, S.; Couturier, J.; Rouhier, N.; Johnson, M.K.; J. Biol. Chem. 293, 4422-4433 (2018)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9LIR4
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
[2Fe-2S]-center
DHAD can accommodate [Fe2S2] and [Fe4S4] clusters. Only the [Fe2S2] cluster-bound form is catalytically active. The [Fe2S2] cluster is coordinated by at least one non-cysteinyl ligand, which can be replaced by the thiol group(s) of dithiothreitol. [Fe2S2] cluster-containing NFU2 protein is likely the physiological cluster donor for in vivo maturation of DHAD
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
[2Fe-2S]-center
DHAD can accommodate [Fe2S2] and [Fe4S4] clusters. Only the [Fe2S2] cluster-bound form is catalytically active. The [Fe2S2] cluster is coordinated by at least one non-cysteinyl ligand, which can be replaced by the thiol group(s) of dithiothreitol. [Fe2S2] cluster-containing NFU2 protein is likely the physiological cluster donor for in vivo maturation of DHAD
Arabidopsis thaliana
Other publictions for EC 4.2.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748233
Gao
Function and maturation of th ...
Arabidopsis thaliana
J. Biol. Chem.
293
4422-4433
2018
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Dihydroxyacid dehydratase is ...
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Studies on the synthesis of th ...
Escherichia coli
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Xing
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Pirrung
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33972
Flint
Dihydroxy acid dehydratase fro ...
Spinacia oleracea
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1988
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33973
Kuo
An activity stain for dihydrox ...
Escherichia coli
Anal. Biochem.
164
526-530
1987
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33984
Smyk-Randall
A reverse-phase high-performan ...
Escherichia coli
Anal. Biochem.
164
434-438
1987
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33974
Armstrong
-
Structure-activity studies wit ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Chem. Soc. Perkin Trans.
1
691-696
1985
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33971
Brown
Dihydroxyacid dehydratase: the ...
Escherichia coli
Biochem. Biophys. Res. Commun.
85
1219-1224
1978
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33970
Armstrong
Stereoselectivity and stereosp ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochim. Biophys. Acta
498
282-293
1977
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33968
Armstrong
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Stereochemistry of the reducto ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Chem. Soc. Chem. Commun.
1974
351-352
1974
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33969
Ryan
Subcellular localization of is ...
Saccharomyces cerevisiae
J. Bacteriol.
120
631-637
1974
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33965
Cassady
Separation of mitochondrial me ...
Neurospora crassa
J. Cell Biol.
53
66-72
1972
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33966
Twarog
Enzymes of the isoleucine-vali ...
Acinetobacter sp. 15150, Acinetobacter sp.
J. Bacteriol.
111
37-46
1972
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33967
Altmiller
Neurospora mutants with mitoch ...
Neurospora crassa
Biochem. Biophys. Res. Commun.
49
1000-1006
1972
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33962
Wixom
A rapid determination of dihyd ...
Escherichia coli, Klebsiella aerogenes
Anal. Biochem.
42
262-274
1971
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33964
Wixom
Studies in valine biosynthesis ...
Achromobacter ruhlandii, Acidovorax facilis, Chromatium sp., Chromatium sp. D, Cupriavidus necator, Paracoccus denitrificans, Rhodobacter sphaeroides, Rhodospirillum rubrum
Biochim. Biophys. Acta
244
532-546
1971
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16
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33961
Altmiller
Purification and properties of ...
Neurospora crassa
Arch. Biochem. Biophys.
138
160-170
1970
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33963
Kiritani
-
alpha,beta-Dihydroxy acid dehy ...
Neurospora crassa, Spinacia oleracea
Methods Enzymol.
17A
755-764
1970
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33960
Kanamori
Studies in valine biosynthesis ...
Spinacia oleracea
J. Biol. Chem.
238
998-1005
1963
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33959
Myers
Dihydroxy acid dehydrase: an e ...
Escherichia coli
J. Biol. Chem.
236
1414-1418
1961
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4
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