Any feedback?
Literature summary for 4.2.1.9 extracted from
- Characterization of recombinantly expressed dihydroxy-acid dehydratase from Sulfobus solfataricus - a key enzyme for the conversion of carbohydrates into chemicals (2015), J. Biotechnol., 211, 31-41.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | increases activity up to 3fold. Activation modulates thermotolerance at 50°C | Saccharolobus solfataricus |  |
| additional information | heat activation: the heat treatment step in purification at 80°C increases the total activity about 2.5 to 3-fold and the specific activity about 20 to 30-fold | Saccharolobus solfataricus |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme can be used for a convenient one-step synthesis route from D-gluconate to 2-dehydro-3-deoxy-D-gluconate | Saccharolobus solfataricus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 (DE3) | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2Fe(SO4)2 | 10 mM, 50°C, 45% loss of activity, dehydration of D-gluconate | Saccharolobus solfataricus | |
| BaCl2 | 100 mM, 50°C, 43% loss of activity, dehydration of D-gluconate | Saccharolobus solfataricus | |
| butanol | - |
Saccharolobus solfataricus | |
| CaCl2 | 10 mM, 50°C, 77% loss of activity, dehydration of D-gluconate | Saccharolobus solfataricus | |
| CuSO4 | 10 mM, 50°C, 86% loss of activity, dehydration of D-gluconate | Saccharolobus solfataricus | |
| D-glycerate | competitive. Presence of 20 mM D-glycerate decreases catalytic activity towards D-gluconate drastically to 5% residual activity | Saccharolobus solfataricus | |
| ethanol | - |
Saccharolobus solfataricus | |
| imidazole | leads to a complete elimination of enzyme activity towards D-gluconate | Saccharolobus solfataricus | |
| isobutanol | - |
Saccharolobus solfataricus | |
| ZnCl2 | 10 mM, 50°C, 95% loss of activity, dehydration of D-gluconate | Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
2,3-Dihydroxy-3-methylbutanoate | pH 7, 50°C | Saccharolobus solfataricus | |
| 7.8 | - |
D-gluconate | pH 7, 50°C | Saccharolobus solfataricus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe-S center | - |
Saccharolobus solfataricus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydroxy-3-methylbutanoate | Saccharolobus solfataricus | biosynthesis pathway of the branched chain amino acids valine and isoleucine | 3-methyl-2-oxobutanoate + H2O | - |
? | |
| 2,3-dihydroxy-3-methylbutanoate | Saccharolobus solfataricus P2 | biosynthesis pathway of the branched chain amino acids valine and isoleucine | 3-methyl-2-oxobutanoate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97UB2 | - |
- |
| Saccharolobus solfataricus P2 | Q97UB2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.7 | - |
pH 7, 50°C, conversion of D-glycerate to pyruvate | Saccharolobus solfataricus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, after purification of His-tagged enzyme the total enzyme activity is decreased to 43% of the crude extract and even further to 17% when stored at -80 °C for one day | Saccharolobus solfataricus |
| -80°C, specific activity for D-gluconate of the frozen/thawed enzyme is stable for 60 min, but loses about 70% after a maximum of 100 min | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydroxy-3-methylbutanoate | - |
Saccharolobus solfataricus | 3-methyl-2-oxobutanoate + H2O | - |
? | |
| 2,3-dihydroxy-3-methylbutanoate | biosynthesis pathway of the branched chain amino acids valine and isoleucine | Saccharolobus solfataricus | 3-methyl-2-oxobutanoate + H2O | - |
? | |
| 2,3-dihydroxy-3-methylbutanoate | - |
Saccharolobus solfataricus P2 | 3-methyl-2-oxobutanoate + H2O | - |
? | |
| 2,3-dihydroxy-3-methylbutanoate | biosynthesis pathway of the branched chain amino acids valine and isoleucine | Saccharolobus solfataricus P2 | 3-methyl-2-oxobutanoate + H2O | - |
? | |
| D-gluconate | - |
Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
| D-gluconate | - |
Saccharolobus solfataricus P2 | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
| D-glycerate | the enzyme catalyzes the non-natural dehydration of D-glycerate to pyruvate | Saccharolobus solfataricus | pyruvate + H2O | - |
? | |
| D-glycerate | the enzyme catalyzes the non-natural dehydration of D-glycerate to pyruvate | Saccharolobus solfataricus P2 | pyruvate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SsDHAD | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 77 | - |
dehydration of D-gluconate | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
half-life: 1028 min | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
D-glycerate | pH 7, 50°C, non-activated enzyme | Saccharolobus solfataricus | |
| 0.03 | - |
D-glycerate | pH 7, 50°C, enzyme activated by 2-mercaptoethanol | Saccharolobus solfataricus | |
| 0.31 | - |
2,3-Dihydroxy-3-methylbutanoate | pH 7, 50°C, enzyme activated by 2-mercaptoethanol | Saccharolobus solfataricus | |
| 0.31 | - |
2,3-Dihydroxy-3-methylbutanoate | pH 7, 50°C, non-activated enzyme | Saccharolobus solfataricus | |
| 0.4 | - |
D-gluconate | pH 7, 50°C, non-activated enzyme | Saccharolobus solfataricus | |
| 1.19 | - |
D-gluconate | pH 7, 50°C, enzyme activated by 2-mercaptoethanol | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
dehydration of D-gluconate | Saccharolobus solfataricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 8.2 | pH 5.2: about 40% of maximal activity, pH 8.2: about 40% of maximal activity, dehydration of D-gluconate | Saccharolobus solfataricus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Fe-S center | - |
Saccharolobus solfataricus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
D-glycerate | 50°C, pH 7 | Saccharolobus solfataricus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| additional information | - |
IC50: 15% (v/v) | Saccharolobus solfataricus | ethanol | |
| additional information | - |
IC50: 4% (v/v) | Saccharolobus solfataricus | isobutanol | |
| additional information | - |
IC50: 4.5% (v/v) | Saccharolobus solfataricus | butanol | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | biosynthesis pathway of the branched chain amino acids valine and isoleucine | Saccharolobus solfataricus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
