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Literature summary for 4.2.1.9 extracted from
- Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production (2010), Appl. Environ. Microbiol., 76, 8053-8061.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | engineering of wild-type Corynebacterium glutamicum for growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production is further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the constructed strains produce up to 188 mM 2-ketoisovalerate and show a product yield of about 0.47 mol per mol of glucose and a volumetric productivity of about 4.6 mM 2-ketoisovalerate per h in the overall production phase, overview | Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-2,3-dihydroxyisovalerate | Corynebacterium glutamicum | - |
2-oxo-isovalerate + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
gene ilvD | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-2,3-dihydroxyisovalerate | - |
Corynebacterium glutamicum | 2-oxo-isovalerate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dihydroxyacid dehydratase | - |
Corynebacterium glutamicum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | dihydroxyacid dehydratase, a key enzyme involved in branched-chain amino acid biosynthesis, catalyses the synthesis of 2-oxoacids from dihydroxyacids | Corynebacterium glutamicum |
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