Literature summary for 4.2.1.9 extracted from

Holatko, J.; Elisakova, V.; Prouza, M.; Sobotka, M.; Nesvera, J.; Patek, M.
Metabolic engineering of the L-valine biosynthesis pathway in Corynebacterium glutamicum using promoter activity modulation (2009), J. Biotechnol., 139, 203-210.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene ilvD, expression in Escherichia coli DH5alpha, overexpression	Corynebacterium glutamicum

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a valine producer by genetic engineering, mutant strains are resistant against inhibition by branched-chain amino acids L-valine, L-isoleucine and L-leucine, A constructed weak promoter of ilvA or leuA, which is introduced into the Corynebacterium glutamicum chromosome via a gene-replacement technique, reduces the biosynthetic rate of isoleucine or leucine, which lowers the mutant growth rate and increases valine production. Overexpression of ilvD driven by the strong mutant promoter P-ilvDM7 resultsin an even higher level of valine production, engineered strains, overview	Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2,3-dihydroxy-3-methylvalerate	Corynebacterium glutamicum	-	3-methyl-2-oxovalerate + H2O	-	?
2,3-dihydroxy-isovalerate	Corynebacterium glutamicum	-	2-oxoisovalerate	-	?

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	-	gene ilvD, strain ilvNM13, ATCC 13032	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,3-dihydroxy-3-methylvalerate	-	Corynebacterium glutamicum	3-methyl-2-oxovalerate + H2O	-	?
2,3-dihydroxy-isovalerate	-	Corynebacterium glutamicum	2-oxoisovalerate	-	?

Synonyms

Synonyms	Comment	Organism
DHAD	-	Corynebacterium glutamicum
dihydroxyacid dehydratase	-	Corynebacterium glutamicum
IlvD	-	Corynebacterium glutamicum

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Release 2023.1 (January 2023)