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Literature summary for 4.2.1.9 extracted from
- Catalytic promiscuity in dihydroxy-acid dehydratase from the thermoacidophilic archaeon Sulfolobus solfataricus (2006), J. Biochem., 139, 591-596.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, 40% inhibition | Saccharolobus solfataricus |  |
| Ca2+ | - |
Saccharolobus solfataricus | |
| Co2+ | - |
Saccharolobus solfataricus | |
| Cu2+ | - |
Saccharolobus solfataricus | |
| Fe2+ | - |
Saccharolobus solfataricus | |
| Mg2+ | - |
Saccharolobus solfataricus | |
| p-chloromercuribenzoic acid | - |
Saccharolobus solfataricus | |
| p-hydroxymercuribenzoic acid | - |
Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.54 | - |
2,3-dihydroxyisovalerate | - |
Saccharolobus solfataricus | |
| 2.42 | - |
D-gluconate | - |
Saccharolobus solfataricus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | enhances activity | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
gel filtration | Saccharolobus solfataricus |
| 66000 | - |
2 * 66000, SDS-PAGE | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Saccharolobus solfataricus | dihydroxy-acid dehydratase is one of the key enzymes involved in the biosynthetic pathway of the branched chain amino acids | ? | - |
? | |
| additional information | Saccharolobus solfataricus P2 | dihydroxy-acid dehydratase is one of the key enzymes involved in the biosynthetic pathway of the branched chain amino acids | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97UB2 | - |
- |
| Saccharolobus solfataricus P2 | Q97UB2 | - |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| when the recombinant enzyme is incubated in 50 mM Tris-HCl buffer at 30°C for 2 h under aerobic or anaerobic conditions and the residual activity is measured, no difference is found between aerobic and anaerobic conditions | Saccharolobus solfataricus |
| when the recombinant enzyme is incubated in 50 mM Tris-HCl buffer at 30°C for 2 h with organic peroxide, the enzyme activity is stable during exposure to 0.5% organic peroxide for 2 h | Saccharolobus solfataricus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dihydroxyisovalerate | - |
Saccharolobus solfataricus | ? | - |
? | |
| D-arabonate | - |
Saccharolobus solfataricus | ? | - |
? | |
| D-arabonate | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
| D-fuconate | - |
Saccharolobus solfataricus | ? | - |
? | |
| D-fuconate | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
| D-Galacturonate | - |
Saccharolobus solfataricus | ? | - |
? | |
| D-gluconate | - |
Saccharolobus solfataricus | 2-keto-3-deoxygluconate + H2O | - |
? | |
| D-Glucuronate | - |
Saccharolobus solfataricus | ? | - |
? | |
| D-Glucuronate | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
| D-xylonate | - |
Saccharolobus solfataricus | ? | - |
? | |
| D-xylonate | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
| additional information | dihydroxy-acid dehydratase is one of the key enzymes involved in the biosynthetic pathway of the branched chain amino acids | Saccharolobus solfataricus | ? | - |
? | |
| additional information | dihydroxy-acid dehydratase is one of the key enzymes involved in the biosynthetic pathway of the branched chain amino acids | Saccharolobus solfataricus P2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 66000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 90 | 60°C: about 60% of maximal activity, 90°C: about 60% of maximal activity | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
2 h, stable | Saccharolobus solfataricus |
| 80 | - |
30 min, 50% loss of activity | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 48.5 | - |
D-gluconate | - |
Saccharolobus solfataricus | |
| 75.8 | - |
2,3-dihydroxyisovalerate | - |
Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Saccharolobus solfataricus |
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