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Literature summary for 4.2.1.9 extracted from

  • Pirrung, M.C.; Holmes, C.P.; Horowitz, D.M.; Nunn, D.S.
    Mechanism and stereochemistry of alpha,beta-dihydroxyacid dehydratase (1991), J. Am. Chem. Soc., 113, 1020-1025.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
(2R,3R)-2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea
0.55
-
2,3-dihydroxybutanoate
-
Spinacia oleracea
0.71
-
(2R,3S)-2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea
0.97
-
2,3-Dihydroxy-3-methylbutanoate
-
Spinacia oleracea
1.78
-
3-cyclopropyl-2,3-dihydroxybutanoate
-
Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O polar but concerted elimination mechanism which should proceed through an anti transition state Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3R)-2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea 3-methyl-2-oxopentanoate + H2O
-
?
(2R,3S)-2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea 3-methyl-2-oxopentanoate + H2O
-
?
2,3-dihydroxy-3-methylbutanoate
-
Spinacia oleracea 2-oxoisovalerate + H2O
-
?
2,3-dihydroxy-3-methylpentanoate 2R,3S-2,3-dihydroxy-3-methylpentanoate, 2R,3R-2,3-dihydroxy-3-methylpentanoate Spinacia oleracea 3-methyl-2-oxopentanoate + H2O
-
?
2,3-dihydroxybutanoate
-
Spinacia oleracea ?
-
?
3-cyclopropyl-2,3-dihydroxybutanoate
-
Spinacia oleracea ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6
-
2,3-dihydroxybutanoate
-
Spinacia oleracea
5.7
-
3-cyclopropyl-2,3-dihydroxybutanoate
-
Spinacia oleracea
8.3
-
(2R,3R)-2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea
11.5
-
2,3-Dihydroxy-3-methylbutanoate
-
Spinacia oleracea
12.3
-
(2R,3R)-2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea