Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong | Methanococcus aeolicus | |
KCN | - |
Methanococcus aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
2,3-dihydroxyisovalerate | - |
Methanococcus aeolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
Fe2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
Mg2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
Mn2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus | |
Ni2+ | divalent cation required. Mg2+, Mn2+, Ni2+, Co2+, and Fe2+ are nearly equally effective | Methanococcus aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxyisovalerate | Methanococcus aeolicus | - |
? | - |
? | |
2,3-dihydroxyisovalerate | Methanococcus maripaludis | - |
? | - |
? | |
2,3-dihydroxyisovalerate | Methanococcus voltae | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus aeolicus | - |
- |
- |
Methanococcus maripaludis | - |
- |
- |
Methanococcus voltae | - |
- |
- |
Oxidation Stability | Organism |
---|---|
5% O2, at 25°C, half-life: less than 6 min, under anaerobic conditions the enzyme is stable for more than 8 days | Methanococcus aeolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.63 | - |
- |
Methanococcus aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxy-3-methylbutanoate | - |
Methanococcus aeolicus | 2-oxoisovalerate + H2O | - |
? | |
2,3-dihydroxy-3-methylbutanoate | - |
Methanococcus maripaludis | 2-oxoisovalerate + H2O | - |
? | |
2,3-dihydroxy-3-methylbutanoate | - |
Methanococcus voltae | 2-oxoisovalerate + H2O | - |
? | |
2,3-dihydroxy-3-methylpentanoate | - |
Methanococcus aeolicus | 3-methyl-2-oxopentanoate + H2O | - |
? | |
2,3-dihydroxy-3-methylpentanoate | - |
Methanococcus maripaludis | 3-methyl-2-oxopentanoate + H2O | - |
? | |
2,3-dihydroxy-3-methylpentanoate | - |
Methanococcus voltae | 3-methyl-2-oxopentanoate + H2O | - |
? | |
2,3-dihydroxyisovalerate | - |
Methanococcus aeolicus | ? | - |
? | |
2,3-dihydroxyisovalerate | - |
Methanococcus maripaludis | ? | - |
? | |
2,3-dihydroxyisovalerate | - |
Methanococcus voltae | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Methanococcus aeolicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 30% of maximal activity, pH 9.0: about 35% of maximal activity | Methanococcus aeolicus |