KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
2,3-dihydroxyisovalerate | pH 8.0, Tris buffer, since only one of the isomers present in the racemic substrate is active, the corrected Km would be 0.75 mM | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains a [4Fe-4S]2+,+ cluster which is essential for catalysis and unstable in the presence of O2 and O2- | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
2 * 66000, SDS-PAGE | Escherichia coli |
125000 | - |
native PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxidative degradation appears to lead to a complete breakdown of the Fe-S cluster, and the resulting protein cannot by reactivated with Fe2+ and thiol reducing agents | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
63 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxy-3-methylbutanoate | - |
Escherichia coli | 2-oxoisovalerate + H2O | - |
? | |
2,3-dihydroxy-3-methylpentanoate | - |
Escherichia coli | 3-methyl-2-oxopentanoate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 66000, SDS-PAGE | Escherichia coli |