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Literature summary for 4.2.1.9 extracted from

  • Kiritani, K.; Wagner, R.P.
    alpha,beta-Dihydroxy acid dehydratase (Neurospora crassa and spinach) (1970), Methods Enzymol., 17A, 755-764.
No PubMed abstract available
Search for more literature for 4.2.1.9

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Neurospora crassa
EDTA
-
 Spinacia oleracea
NaF
-
 Spinacia oleracea
PCMB
-
 Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 2,3-dihydroxyisovalerate
-
 Spinacia oleracea
6.3
-
 2,3-dihydroxy-3-methylpentanoate
-
 Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Spinacia oleracea
Mg2+ required Neurospora crassa
Mg2+ required for maximal activity, maximal activity at 0.01 M Spinacia oleracea
Mn2+ activates Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Neurospora crassa
-
 Spinacia oleracea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.18
-
-
 Spinacia oleracea
10.13
-
-
 Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxy-3-methylbutanoate
-
 Neurospora crassa 2-oxoisovalerate + H2O
-
 ?
2,3-dihydroxy-3-methylbutanoate
-
 Spinacia oleracea 2-oxoisovalerate + H2O
-
 ?
2,3-dihydroxy-3-methylpentanoate
-
 Neurospora crassa 3-methyl-2-oxopentanoate + H2O
-
 ?
2,3-dihydroxy-3-methylpentanoate
-
 Spinacia oleracea 3-methyl-2-oxopentanoate + H2O
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7 8 2,3-dihydroxy-3-methylpentanoate Neurospora crassa
8 8.2 2,3-dihydroxy-3-methylpentanoate, 2,3-dihydroxyisovalerate Spinacia oleracea
8 8.3 2,3-dihydroxyisovalerate Neurospora crassa