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Literature summary for 4.2.1.9 extracted from

  • Kanamori, M.; Wixom, R.L.
    Studies in valine biosynthesis. V. Characteristics of the purified dihydroxyacid dehydratase from spinach leaves (1963), J. Biol. Chem., 238, 998-1005.
    View publication on PubMed

General Stability

General Stability Organism
enzyme in crude extract is labile to heat and acid Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Spinacia oleracea
Cu2+ weak Spinacia oleracea
Cys
-
Spinacia oleracea
EDTA
-
Spinacia oleracea
Hg2+
-
Spinacia oleracea
NaF
-
Spinacia oleracea
NEM
-
Spinacia oleracea
PCMB
-
Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
2,3-dihydroxyisovalerate
-
Spinacia oleracea
6.3
-
2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Spinacia oleracea 5739
-
soluble
-
Spinacia oleracea
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Spinacia oleracea
Mg2+ required for maximal activity, maximal activity at 0.01 M Spinacia oleracea
Mn2+ activates Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3-dihydroxy-3-methylpentanoate Spinacia oleracea involved in synthesis of Ile ?
-
?
2,3-dihydroxyisovalerate Spinacia oleracea involved in biosynthesis of Val ?
-
?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxy-3-methylbutanoate
-
Spinacia oleracea 2-oxoisovalerate + H2O
-
?
2,3-dihydroxy-3-methylpentanoate
-
Spinacia oleracea 3-methyl-2-oxopentanoate + H2O
-
?
2,3-dihydroxy-3-methylpentanoate involved in synthesis of Ile Spinacia oleracea ?
-
?
2,3-dihydroxyisovalerate involved in biosynthesis of Val Spinacia oleracea ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.2 2,3-dihydroxy-3-methylpentanoate, 2,3-dihydroxyisovalerate Spinacia oleracea