General Stability | Organism |
---|---|
unstable during purification, probably because of dissociation into inactive protein compounds | Eubacterium barkeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | 1 mM: 97% inhibition | Eubacterium barkeri | |
EDTA | 1-5 mM: 70% loss of activity | Eubacterium barkeri | |
additional information | 10 min, 25°C, 2 mM iodoacetamide or iodoacetate: no influence | Eubacterium barkeri | |
N-ethylmaleimide | 1 mM: 93% inhibition | Eubacterium barkeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
dimethylmaleate | 35°C, pH 7 | Eubacterium barkeri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Eubacterium barkeri | |
additional information | Fe2+ not replaceable by Ca2+, Mg2+, Mn2+ or Zn2+ | Eubacterium barkeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
153000 | - |
gel filtration | Eubacterium barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylmaleate + H2O | Eubacterium barkeri | - |
(2R,3S)-2,3-dimethylmalate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eubacterium barkeri | - |
- |
- |
Oxidation Stability | Organism |
---|---|
oxygen-sensitive | Eubacterium barkeri |
Purification (Comment) | Organism |
---|---|
ATCC 25849, partial | Eubacterium barkeri |
Renatured (Comment) | Organism |
---|---|
nearly completetly inactivated enzyme may be reactivated by addition of FeSO4 and glutathione, providing reducing conditions | Eubacterium barkeri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.14 | - |
- |
Eubacterium barkeri |
Storage Stability | Organism |
---|---|
-20°C, 45% loss of activity within 1 day | Eubacterium barkeri |
4°C, 85% loss of activity within 1 day | Eubacterium barkeri |
liquid N2, enzyme extract as well as crude extract stable for several weeks by addition of 20 mM citraconate and 4 mM dithiothreitol, 20 mM potassium phosphate, pH 7.0 | Eubacterium barkeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylmaleate + H2O | - |
Eubacterium barkeri | (2R,3S)-2,3-dimethylmalate | - |
r | |
dimethylmaleate + H2O | equilibrium 70% in favour of 2,3-dimethylmalate, dimethylfumarate is no substrate | Eubacterium barkeri | (2R,3S)-2,3-dimethylmalate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Eubacterium barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | broad | Eubacterium barkeri |