BRENDA - Enzyme Database
show all sequences of 4.2.1.85

Nicotinic acid metabolism. Dimethylmaleate hydratase

Kollmann-Koch, A.; Eggerer, H.; Hoppe-Seyler's Z. Physiol. Chem. 365, 847-857 (1984)

Data extracted from this reference:

General Stability
General Stability
Organism
unstable during purification, probably because of dissociation into inactive protein compounds
Eubacterium barkeri
Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
1 mM: 97% inhibition
Eubacterium barkeri
EDTA
1-5 mM: 70% loss of activity
Eubacterium barkeri
additional information
10 min, 25C, 2 mM iodoacetamide or iodoacetate: no influence
Eubacterium barkeri
N-ethylmaleimide
1 mM: 93% inhibition
Eubacterium barkeri
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1
-
dimethylmaleate
35C, pH 7
Eubacterium barkeri
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Eubacterium barkeri
additional information
Fe2+ not replaceable by Ca2+, Mg2+, Mn2+ or Zn2+
Eubacterium barkeri
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
153000
-
gel filtration
Eubacterium barkeri
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dimethylmaleate + H2O
Eubacterium barkeri
-
(2R,3S)-2,3-dimethylmalate
-
Eubacterium barkeri
r
Organism
Organism
UniProt
Commentary
Textmining
Eubacterium barkeri
-
-
-
Oxidation Stability
Oxidation Stability
Organism
oxygen-sensitive
Eubacterium barkeri
Purification (Commentary)
Purification (Commentary)
Organism
ATCC 25849, partial
Eubacterium barkeri
Renatured (Commentary)
Renatured (Commentary)
Organism
nearly completetly inactivated enzyme may be reactivated by addition of FeSO4 and glutathione, providing reducing conditions
Eubacterium barkeri
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.14
-
-
Eubacterium barkeri
Storage Stability
Storage Stability
Organism
-20C, 45% loss of activity within 1 day
Eubacterium barkeri
4C, 85% loss of activity within 1 day
Eubacterium barkeri
liquid N2, enzyme extract as well as crude extract stable for several weeks by addition of 20 mM citraconate and 4 mM dithiothreitol, 20 mM potassium phosphate, pH 7.0
Eubacterium barkeri
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
dimethylmaleate + H2O
-
648458
Eubacterium barkeri
(2R,3S)-2,3-dimethylmalate
-
648458
Eubacterium barkeri
r
dimethylmaleate + H2O
equilibrium 70% in favour of 2,3-dimethylmalate, dimethylfumarate is no substrate
648458
Eubacterium barkeri
(2R,3S)-2,3-dimethylmalate
-
648458
Eubacterium barkeri
r
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
assay at
Eubacterium barkeri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
9
broad
Eubacterium barkeri
General Stability (protein specific)
General Stability
Organism
unstable during purification, probably because of dissociation into inactive protein compounds
Eubacterium barkeri
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
1 mM: 97% inhibition
Eubacterium barkeri
EDTA
1-5 mM: 70% loss of activity
Eubacterium barkeri
additional information
10 min, 25C, 2 mM iodoacetamide or iodoacetate: no influence
Eubacterium barkeri
N-ethylmaleimide
1 mM: 93% inhibition
Eubacterium barkeri
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1
-
dimethylmaleate
35C, pH 7
Eubacterium barkeri
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required
Eubacterium barkeri
additional information
Fe2+ not replaceable by Ca2+, Mg2+, Mn2+ or Zn2+
Eubacterium barkeri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
153000
-
gel filtration
Eubacterium barkeri
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dimethylmaleate + H2O
Eubacterium barkeri
-
(2R,3S)-2,3-dimethylmalate
-
Eubacterium barkeri
r
Oxidation Stability (protein specific)
Oxidation Stability
Organism
oxygen-sensitive
Eubacterium barkeri
Purification (Commentary) (protein specific)
Commentary
Organism
ATCC 25849, partial
Eubacterium barkeri
Renatured (Commentary) (protein specific)
Commentary
Organism
nearly completetly inactivated enzyme may be reactivated by addition of FeSO4 and glutathione, providing reducing conditions
Eubacterium barkeri
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.14
-
-
Eubacterium barkeri
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 45% loss of activity within 1 day
Eubacterium barkeri
4C, 85% loss of activity within 1 day
Eubacterium barkeri
liquid N2, enzyme extract as well as crude extract stable for several weeks by addition of 20 mM citraconate and 4 mM dithiothreitol, 20 mM potassium phosphate, pH 7.0
Eubacterium barkeri
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
dimethylmaleate + H2O
-
648458
Eubacterium barkeri
(2R,3S)-2,3-dimethylmalate
-
648458
Eubacterium barkeri
r
dimethylmaleate + H2O
equilibrium 70% in favour of 2,3-dimethylmalate, dimethylfumarate is no substrate
648458
Eubacterium barkeri
(2R,3S)-2,3-dimethylmalate
-
648458
Eubacterium barkeri
r
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
assay at
Eubacterium barkeri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
9
broad
Eubacterium barkeri
Other publictions for EC 4.2.1.85
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
648458
Kollmann-Koch
Nicotinic acid metabolism. Dim ...
Eubacterium barkeri
Hoppe-Seyler's Z. Physiol. Chem.
365
847-857
1984
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4
1
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2
1
1
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3
1
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1
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1
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1
3
2
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1
-
-
-
1
-
-
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-
-
-
-
-
-
-
-
1
-
4
-
1
-
2
1
1
-
1
-
1
1
-
1
3
2
-
1
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1
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