Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.84 extracted from

  • Cheng, Z.; Jiang, S.; Zhou, Z.
    Substrate access tunnel engineering for improving the catalytic activity of a thermophilic nitrile hydratase toward pyridine and pyrazine nitriles (2021), Biochem. Biophys. Res. Commun., 575, 8-13.
    View publication on PubMed
Search for more literature for 4.2.1.84

Protein Variants

Protein Variants Comment Organism
L48H mutation of residue located at the substrate access tunnel of subunit NthB, specific activities towards 3-cyanopyridine, 2-cyanopyridine and cyanopyrazine are 2.4fold, 2.8fold and 3.1fold higher tha that of wild-type. Introduced H48 can form a long-lasting hydrogen bond with E166 of sub unit NthA, which contributes to the expansion of the entrance of substrate access tunnel Caldalkalibacillus thermarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 3-Cyanopyridine mutant L48H, pH 7.4, 30°C Caldalkalibacillus thermarum
1.2
-
 3-Cyanopyridine wild-type, pH 7.4, 30°C Caldalkalibacillus thermarum

Organism

Organism UniProt Comment Textmining
Caldalkalibacillus thermarum F5L3T2 and F5L3T3 F5L3T2 i.e. subunit NthA, F5L3T3 i.e. subunit NthB
-
Caldalkalibacillus thermarum TA2. A1 F5L3T2 and F5L3T3 F5L3T2 i.e. subunit NthA, F5L3T3 i.e. subunit NthB
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
217.2
-
 wild-type, substrate 2-cyanopyridine, pH 7.4, 30°C Caldalkalibacillus thermarum
225.9
-
 wild-type, substrate cyanopyrazine, pH 7.4, 30°C Caldalkalibacillus thermarum
383.8
-
 wild-type, substrate 3-cyanopyridine, pH 7.4, 30°C Caldalkalibacillus thermarum
608.2
-
 mutant L48H, substrate 2-cyanopyridine, pH 7.4, 30°C Caldalkalibacillus thermarum
690.5
-
 mutant L48H, substrate cyanopyrazine, pH 7.4, 30°C Caldalkalibacillus thermarum
902.3
-
 mutant L48H, substrate 3-cyanopyridine, pH 7.4, 30°C Caldalkalibacillus thermarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-cyanopyridine + H2O
-
 Caldalkalibacillus thermarum pyridine-2-carbamide
-
 ?
2-cyanopyridine + H2O
-
 Caldalkalibacillus thermarum TA2. A1 pyridine-2-carbamide
-
 ?
3-cyanopyridine + H2O
-
 Caldalkalibacillus thermarum nicotinamide
-
 ?
3-cyanopyridine + H2O
-
 Caldalkalibacillus thermarum TA2. A1 nicotinamide
-
 ?
cyanopyrazine + H2O
-
 Caldalkalibacillus thermarum pyrazincarbamide
-
 ?
cyanopyrazine + H2O
-
 Caldalkalibacillus thermarum TA2. A1 pyrazincarbamide
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
352.1
-
 3-Cyanopyridine wild-type, pH 7.4, 30°C Caldalkalibacillus thermarum
833.2
-
 3-Cyanopyridine mutant L48H, pH 7.4, 30°C Caldalkalibacillus thermarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
293.4
-
 3-Cyanopyridine wild-type, pH 7.4, 30°C Caldalkalibacillus thermarum
1388.7
-
 3-Cyanopyridine mutant L48H, pH 7.4, 30°C Caldalkalibacillus thermarum