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Literature summary for 4.2.1.84 extracted from

  • Lan, Y.; Zhang, X.; Liu, Z.; Zhou, L.; Shen, R.; Zhong, X.; Cui, W.; Zhou, Z.
    Overexpression and characterization of two types of nitrile hydratases from Rhodococcus rhodochrous J1 (2017), PLoS ONE, 12, e0179833 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.84

Application

Application Comment Organism
industry biotransformation of nitrile. Nitrile hydratase from Rhodococcus rhodochrous J1 is used for industrial production of acrylamide and nicotinamide. Production of enzyme by recombinant Escherichia coli is superior to that in R. rhodochrous J1. Genetically engineered Escherichia coli can be used for industrial applications instead of Rhodococcus rhodochrous J1. High-molecular weight nitrile hydratase may be more suitable for industrial application than low-molecular weight nitrile hydratase because of its higher product tolerance, which would lead to a high product concentration Rhodococcus rhodochrous

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli by codon-optimization, engineering of Ribosome Binding Site and spacer sequences Rhodococcus rhodochrous

Inhibitors

Inhibitors Comment Organism Structure
3-Cyanopyridine 1 M, 40% decrease of activity, low-molecular weight nitrile hydratase; high-molecular weight nitrile hydratase show almost no inhibition at 1 M Rhodococcus rhodochrous
nicotinamide 0.5 M, sharp decrease in activity, low-molecular weight nitrile hydratase; 1.5 M, 40% decrease of activity, high-molecular weight nitrile hydratase Rhodococcus rhodochrous

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ about 1 mol of cobalt per mol of enzyme Rhodococcus rhodochrous

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
 gel filtration Rhodococcus rhodochrous
504000
-
 gel filtration Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous P21219 AND P21220 P21219: alpha-subunit, P21220: beta-subunit
-
Rhodococcus rhodochrous P29378 AND P29379 P29378: alpha-subunit, P29379: beta-subunit
-
Rhodococcus rhodochrous J1 P21219 AND P21220 P21219: alpha-subunit, P21220: beta-subunit
-
Rhodococcus rhodochrous J1 P29378 AND P29379 P29378: alpha-subunit, P29379: beta-subunit
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyanopyridine + H2O
-
 Rhodococcus rhodochrous pyridine-3-carbamide
-
 ?
3-cyanopyridine + H2O
-
 Rhodococcus rhodochrous J1 pyridine-3-carbamide
-
 ?

Subunits

Subunits Comment Organism
oligomer alpha10beta10, 10 * 22800 (alpha) + 10 * 26300 (beta), calculated from sequence Rhodococcus rhodochrous
tetramer alpha2beta2, 2 * 22800 (alpha) + 2 * 25200 (beta), calculated from sequence Rhodococcus rhodochrous

Synonyms

Synonyms Comment Organism
H-NHase
-
 Rhodococcus rhodochrous
high-molecular weight nitrile hydratase
-
 Rhodococcus rhodochrous
L-Nhase
-
 Rhodococcus rhodochrous
low-molecular weight nitrile hydratase
-
 Rhodococcus rhodochrous

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Rhodococcus rhodochrous

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, 60% loss of activity high-molecular weight nitrile hydratase Rhodococcus rhodochrous
50
-
 40 min, 40% loss of activity low-molecular weight nitrile hydratase Rhodococcus rhodochrous

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Rhodococcus rhodochrous