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Literature summary for 4.2.1.84 extracted from
- Analyzing the catalytic role of active site residues in the Fe-type nitrile hydratase from Comamonas testosteroni Ni1 (2015), J. Biol. Inorg. Chem., 20, 885-894 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Comamonas testosteroni |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, X-ray crystal structures of the alphaH80A/alphaH81A, alphaH80W/alphaH81W, and alphaR157A mutant CtNHase enzymes are solved to 2.0, 2.8, and 2.5 A resolutions, respectively | Comamonas testosteroni |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H80A | mutant of alpha-subunit, activity (kcat = 220/s) accounts for about 20% of the wild-type activity (kcat = 1100/s) while the Km value is slightly reduced (187 mM) | Comamonas testosteroni |
| H80A/H81A | mutant of alpha-subunit, activity (kcat = 132/s) accounts for 12% of the wild-type activity (kcat = 1100/s) while the Km value is nearly unchanged at 205 mM. Hydrogen-bonding interactions crucial for the catalytic function of the alphaCys104-SOH ligand are disrupted. Disruption of these hydrogen bonding interactions likely alters the nucleophilicity of the sulfenic acid oxygen and the Lewis acidity of the active site Fe(III) ion | Comamonas testosteroni |
| H80W/H81W | mutant of alpha-subunit, disruption of these hydrogen bonding interactions likely alters the nucleophilicity of the sulfenic acid oxygen and the Lewis acidity of the active site Fe(III) ion | Comamonas testosteroni |
| H81A | mutant of alpha-subunit, activity (kcat = 77/s) accounts for 4% of the wild-type activity (kcat = 1100/s) while the Km value is slightly reduced (179 mM) | Comamonas testosteroni |
| R157A | mutant of alpha-subunit, activity (kcat = 10/s) accounts for less than 1% of the wild-type activity (kcat = 1100/s) while the Km value is nearly unchanged at 205 mM | Comamonas testosteroni |
| R157A | mutant of alpha-subunit, disruption of these hydrogen bonding interactions likely alters the nucleophilicity of the sulfenic acid oxygen and the Lewis acidity of the active site Fe(III) ion | Comamonas testosteroni |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 179 | - |
acrylonitrile | pH 7.0, 25°C, alpha-subunit mutant enzyme H81A | Comamonas testosteroni |  |
| 187 | - |
acrylonitrile | pH 7.0, 25°C, alpha-subunit mutant enzyme H80A | Comamonas testosteroni | |
| 204 | - |
acrylonitrile | pH 7.0, 25°C, alpha-subunit mutant enzyme R157A | Comamonas testosteroni | |
| 213 | - |
acrylonitrile | pH 7.0, 25°C, alpha-subunit mutant enzyme H80A/H81A | Comamonas testosteroni | |
| 232 | - |
acrylonitrile | pH 7.0, 25°C, alpha-subunit mutant enzyme H80W/H81W | Comamonas testosteroni | |
| 239 | - |
acrylonitrile | pH 7.0, 25°C, alpha-subunit mutant enzyme R157K | Comamonas testosteroni | |
| 250 | - |
acrylonitrile | pH 7.0, 25°C, wild-type enzyme | Comamonas testosteroni | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | Fe-type nitrile hydratase. Iron content of wild-type enzyme is 1.4 equivalents of iron per (alphabeta)2 heterotetramer. Mutating the active site alphaH80, alphaHs81, and alphaR157 residues have little or no effect on the incorporation of iron into the active site. In the alpha-subunit mutant enzyme (H80W/H81W, H80A/H81A and R157A), hydrogen-bonding interactions crucial for the catalytic function of the alphaCys104-SOH ligand are disrupted. Disruption of these hydrogen bonding interactions likely alters the nucleophilicity of the sulfenic acid oxygen and the Lewis acidity of the active site Fe(III) ion | Comamonas testosteroni | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Comamonas testosteroni | J9PBS0 AND J9PBS1 | J9PBS0: alpha-subunit, J9PBS1: beta-subunit | - |
| Comamonas testosteroni Ni1 | J9PBS0 AND J9PBS1 | J9PBS0: alpha-subunit, J9PBS1: beta-subunit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Comamonas testosteroni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acrylonitrile + H2O | - |
Comamonas testosteroni | ? | - |
? | |
| acrylonitrile + H2O | - |
Comamonas testosteroni Ni1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | - |
Comamonas testosteroni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CtNHase | - |
Comamonas testosteroni |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
acrylonitrile | pH 7.0, 25°C, alpha-subunit mutant enzyme R157A | Comamonas testosteroni | |
| 32 | - |
acrylonitrile | pH 7.0, 25°C, alpha-subunit mutant enzyme R157K | Comamonas testosteroni | |
| 77 | - |
acrylonitrile | pH 7.0, 25°C, alpha-subunit mutant enzyme H81A | Comamonas testosteroni | |
| 79 | - |
acrylonitrile | pH 7.0, 25°C, alpha-subunit mutant enzyme H80W/H81W | Comamonas testosteroni | |
| 132 | - |
acrylonitrile | pH 7.0, 25°C, alpha-subunit mutant enzyme H80A/H81A | Comamonas testosteroni | |
| 220 | - |
acrylonitrile | pH 7.0, 25°C, alpha-subunit mutant enzyme H80A | Comamonas testosteroni | |
| 1100 | - |
acrylonitrile | pH 7.0, 25°C, wild-type enzyme | Comamonas testosteroni | |
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