Application | Comment | Organism |
---|---|---|
synthesis | industrial production of highly purified acrylamide and nicotinamide. The thermostability and catalytic efficiency of the subunit-fused nitrile hydratase is improved by semi-rational engineering | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
M150C | beta-subunit mutant enzyme, 32% increase in half-life at 50°C, the kcat/Km value is 1.1fold higher than the kcat/Km value of the wild-type enzyme | Pseudomonas putida |
S189E | beta-subunit mutant enzyme, 107% increase in half-life at 50°C, the kcat/Km value is 2.2fold higher than the kcat/Km value of the wild-type enzyme | Pseudomonas putida |
T173Y | beta-subunit mutant enzyme, 7% increase in half-life at 50°C, the kcat/Km value is 1.5fold higher than the kcat/Km value of the wild-type enzyme | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
36.3 | - |
3-Cyanopyridine | 25°C, pH not specified in the publication, beta-subunit mutant enzyme S189E | Pseudomonas putida | |
62.9 | - |
3-Cyanopyridine | 25°C, pH not specified in the publication, subunit-fused wild-type nitrile hydratase | Pseudomonas putida | |
64.9 | - |
3-Cyanopyridine | 25°C, pH not specified in the publication, beta-subunit mutant enzyme T173Y | Pseudomonas putida | |
72.8 | - |
3-Cyanopyridine | 25°C, pH not specified in the publication, beta-subunit mutant enzyme M150C | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P97051 AND P97052 | P97051: alpha-subunit, P97052: beta-subunit | - |
Pseudomonas putida NRRL-18668 | P97051 AND P97052 | P97051: alpha-subunit, P97052: beta-subunit | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyanopyridine + H2O | - |
Pseudomonas putida | pyridine-3-carboxamide | - |
? | |
3-cyanopyridine + H2O | - |
Pseudomonas putida NRRL-18668 | pyridine-3-carboxamide | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
t1/2: 26 min (subunit-fused wild-type nitrile hydratase), 36 min (beta-subunit mutant enzyme A119D), 32 min (beta-subunit mutant enzyme V139A), 34 min (beta-subunit mutant enzyme N141G), 13 min (beta-subunit mutant enzyme T148G), 32 min (beta-subunit mutant enzyme R149G), 40 min (beta-subunit mutant enzyme M150C), 29 min (beta-subunit mutant enzyme G156M), 30 min (beta-subunit mutant enzyme G156S), 25 min (beta-subunit mutant enzyme G156Q), 24 min (beta-subunit mutant enzyme G156N), 32 min (beta-subunit mutant enzyme G156E), 23 min (beta-subunit mutant enzyme H165G), 32 min (beta-subunit mutant enzyme T173Y), 38 min (beta-subunit mutant enzyme T173F), 24 min (beta-subunit mutant enzyme V188G), above 60 min (beta-subunit mutant enzyme S189E), 28 min (beta-subunit mutant enzyme Q198G) | Pseudomonas putida |
55 | - |
Tm value of subunit-fused wild-type nitrile hydratase is 54.5°C, Tm value of beta-subunit mutant enzyme T173Y is 55°C | Pseudomonas putida |
56 | - |
Tm value of beta-subunit mutant enzyme M150C is 56.2°C | Pseudomonas putida |
58 | - |
Tm value of beta-subunit mutant enzyme S189E | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
720.6 | - |
3-Cyanopyridine | 25°C, pH not specified in the publication, subunit-fused wild-type nitrile hydratase | Pseudomonas putida | |
922.3 | - |
3-Cyanopyridine | 25°C, pH not specified in the publication, beta-subunit mutant enzyme M150C | Pseudomonas putida | |
1112 | - |
3-Cyanopyridine | 25°C, pH not specified in the publication, beta-subunit mutant enzyme T173Y | Pseudomonas putida | |
8935.6 | - |
3-Cyanopyridine | 25°C, pH not specified in the publication, beta-subunit mutant enzyme S189E | Pseudomonas putida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11.5 | - |
3-Cyanopyridine | 25°C, pH not specified in the publication, subunit-fused wild-type nitrile hydratase | Pseudomonas putida | |
12.7 | - |
3-Cyanopyridine | 25°C, pH not specified in the publication, beta-subunit mutant enzyme M150C | Pseudomonas putida | |
17.1 | - |
3-Cyanopyridine | 25°C, pH not specified in the publication, beta-subunit mutant enzyme T173Y | Pseudomonas putida | |
25.8 | - |
3-Cyanopyridine | 25°C, pH not specified in the publication, beta-subunit mutant enzyme S189E | Pseudomonas putida |