Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodococcus rhodochrous |
Protein Variants | Comment | Organism |
---|---|---|
A51L | wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 74.3% | Rhodococcus rhodochrous |
F37H | wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 96.8% | Rhodococcus rhodochrous |
F37H/F51L | wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 94.6% | Rhodococcus rhodochrous |
F37H/L48A | wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 95.1% | Rhodococcus rhodochrous |
F37Y | wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 88.7% | Rhodococcus rhodochrous |
F51Q | wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 60.2% | Rhodococcus rhodochrous |
L48Q | wild-type enzyme catalyzes the conversion of (S)-mandelamide with an enantiomeric excess of 52.6%, the beta-subunit mutant enzyme catalyzes the reaction with an enantiomeric excess of 59.7% | Rhodococcus rhodochrous |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38.2 | - |
(S)-mandelonitrile | pH 7.4, 10°C, wild-type enzyme | Rhodococcus rhodochrous | |
41.9 | - |
(S)-mandelonitrile | pH 7.4, 10°C, mutant enzyme F37H | Rhodococcus rhodochrous | |
43.8 | - |
(R)-mandelonitrile | pH 7.4, 10°C, wild-type enzyme | Rhodococcus rhodochrous |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mandelonitrile + H2O | Rhodococcus rhodochrous | wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% | (R)-mandelamide | - |
? | |
(R)-mandelonitrile + H2O | Rhodococcus rhodochrous J1 | wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% | (R)-mandelamide | - |
? | |
(S)-mandelonitrile + H2O | Rhodococcus rhodochrous | wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% | (S)-mandelamide | - |
? | |
(S)-mandelonitrile + H2O | Rhodococcus rhodochrous J1 | wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% | (S)-mandelamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | P29378 AND P29379 | P29378: alpha-subunit, P29379: beta-subunit | - |
Rhodococcus rhodochrous J1 | P29378 AND P29379 | P29378: alpha-subunit, P29379: beta-subunit | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus rhodochrous |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mandelonitrile + H2O | wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% | Rhodococcus rhodochrous | (R)-mandelamide | - |
? | |
(R)-mandelonitrile + H2O | wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% | Rhodococcus rhodochrous J1 | (R)-mandelamide | - |
? | |
(S)-mandelonitrile + H2O | wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% | Rhodococcus rhodochrous | (S)-mandelamide | - |
? | |
(S)-mandelonitrile + H2O | wild-type enzyme catalyzes the conversion of rac-mandelonitrile to (S)-mandelamide with an enantiomeric excess of 52.6% | Rhodococcus rhodochrous J1 | (S)-mandelamide | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
(R)-mandelonitrile | pH 7.4, 10°C, wild-type enzyme | Rhodococcus rhodochrous | |
1.2 | - |
(S)-mandelonitrile | pH 7.4, 10°C, wild-type enzyme | Rhodococcus rhodochrous | |
1.4 | - |
(S)-mandelonitrile | pH 7.4, 10°C, mutant enzyme F37H | Rhodococcus rhodochrous |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0168 | - |
(R)-mandelonitrile | pH 7.4, 10°C, wild-type enzyme | Rhodococcus rhodochrous | |
0.032 | - |
(S)-mandelonitrile | pH 7.4, 10°C, wild-type enzyme | Rhodococcus rhodochrous | |
0.034 | - |
(S)-mandelonitrile | pH 7.4, 10°C, mutant enzyme F37H | Rhodococcus rhodochrous |