Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type enzyme and peptide fused enzyme variants in Escherichia coli strain BL21(DE3) | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the thermostability and product tolerance of nitrile hydratase are enhanced by fusing with two of the self-assembling amphipathic peptides, EAK16 (AEAEAKAKAEAEAKAK) at the N- and C-terminus and ELK16 (LELELKLKLELELKLK) at the N-terminus. When self-assembling amphipathic peptide ELK16 is fused to the N-terminus of the enzymes beta-subunit, the resultant enzyme (SAP-NHase-2) becomes an active inclusion body, while EAK16 does not affect the enzyme solubility when fused to the enzyme's C-terminus (SAP-NHase-10) or N-termninus (AP-NHase-1) of the beta-subunit | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acrylamide | NHases exhibit low product acrylamide tolerance | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida NRRL-18668 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type enzyme and peptide fused enzyme variants from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13 | - |
cell extract supernatant containing recombinant His-tagged ELK-16 N-terminal fusion enzyme variant, pH 7.5, 20°C | Pseudomonas putida |
109 | - |
cell extract supernatant containing recombinant His-tagged EAK-16 N-terminal fusion enzyme variant, pH 7.5, 20°C | Pseudomonas putida |
110 | - |
cell extract supernatant containing recombinant His-tagged wild-type enzyme, pH 7.5, 20°C | Pseudomonas putida |
372 | - |
purified recombinant His-tagged ELK-16 N-terminal fusion enzyme variant, pH 7.5, 20°C | Pseudomonas putida |
426 | - |
purified recombinant His-tagged EAK-16 N-terminal fusion enzyme variant, pH 7.5, 20°C | Pseudomonas putida |
429 | - |
purified recombinant His-tagged EAK-16 C-terminal fusion enzyme variant, pH 7.5, 20°C | Pseudomonas putida |
439 | - |
purified recombinant His-tagged wild-type enzyme, pH 7.5, 20°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-cyanopyridine + H2O | - |
Pseudomonas putida | pyridine-3-carbamide | i.e. nicotinamide | ? | |
3-cyanopyridine + H2O | - |
Pseudomonas putida NRRL-18668 | pyridine-3-carbamide | i.e. nicotinamide | ? | |
acrylonitrile + H2O | - |
Pseudomonas putida | acrylamide | - |
? | |
acrylonitrile + H2O | - |
Pseudomonas putida NRRL-18668 | acrylamide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NHase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
wild-type enzyme, 30 min, deactivation, enzyme fused to self-assembling amphipathic peptide ELK16 (LELELKLKLELELKLK) retains 30% activity, and SAP-NHase-1 N-terminally fused to self-assembling amphipathic peptide EAK16 (AEAEAKAKAEAEAKAK) retains 45% activity, C-terminally fused enzyme SAP-NHase-10 50% activity. Treatment with buffer containing 10% acrylamide increases the thermostability so that the wild-type retains 30% activity, and the fused enzymes SAP-NHase-1, SAP-NHase-2, and SAP-NHase-10 retain 52%, 42%, and 55% activity, respectively | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pseudomonas putida |