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Literature summary for 4.2.1.84 extracted from
- Identification of an active site-bound nitrile hydratase intermediate through single turnover stopped-flow spectroscopy (2013), J. Biol. Chem., 288, 15532-15536.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Rhodococcus equi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | single turnover stopped-flow experiments and multiple turnover experiments with methacrylonitrile suggest a three-step kinetic model that allows for the reversible binding of substrate, the presence of an intermediate, and the formation of product, identification of catalytic Fe3+-nitrile intermediate species, kinetics, detaiiled overview | Rhodococcus equi |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | iron-type nitrile hydratase | Rhodococcus equi |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus equi | - |
- |
- |
| Rhodococcus equi TG328-2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography and ultrafiltration | Rhodococcus equi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methacrylonitrile + H2O | - |
Rhodococcus equi | methacrylamide | - |
? | |
| methacrylonitrile + H2O | - |
Rhodococcus equi TG328-2 | methacrylamide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| iron-type nitrile hydratase | - |
Rhodococcus equi |
| NHase | - |
Rhodococcus equi |
| ReNHase | - |
Rhodococcus equi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rhodococcus equi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Rhodococcus equi |
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Release 2023.1 (January 2023)
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