Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.84 extracted from

  • Duca, D.; Rose, D.R.; Glick, B.R.
    Characterization of a nitrilase and a nitrile hydratase from Pseudomonas sp. UW4 that converts indole-3-acetonitrile to produce indole-3-acetic acid (2014), Appl. Environ. Microbiol., 80, 4640-4649.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.84

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme does not require the help of a P47K activator protein to be active Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene nthAB, DNA and amino acid sequence determination, analysis, and comparison, phylogenetic analysis and tree, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ iron-type metalloenzyme Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
indole-3-nitrile + H2O Pseudomonas sp.
-
 indole-3-acetamide the nitrile hydratase produces only indole-3-acetamide, no indole-3-acetic acid ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. K9NKC3 gene nthA, alpha-subunit; gene nthAB
-
Pseudomonas sp. K9NLN7 gene nthB, beta-subunit; gene nthAB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition optimum growth temperature of the bacterium is 25°C to 30°C, but it is able to grow at 4°C Pseudomonas sp.
-
additional information optimum growth temperature of the bacterium is 25°C to 30°C, but it is able to grow at 4°C Pseudomonas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
indole-3-nitrile + H2O
-
 Pseudomonas sp. indole-3-acetamide
-
 ?
indole-3-nitrile + H2O
-
 Pseudomonas sp. indole-3-acetamide the nitrile hydratase produces only indole-3-acetamide, no indole-3-acetic acid ?

Subunits

Subunits Comment Organism
heterodimer alphabeta, nitrile hydratase alpha (NthA), and beta (NthB) subunits Pseudomonas sp.

Synonyms

Synonyms Comment Organism
NthAB
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 maximal activity at 4°C, over 50% of maximal activity at temperatures of 10 to 20°C, the activity decreases significantly as the temperature increases above 20°C Pseudomonas sp.
-
 20 maximal activity at 4°C, over 50% of maximal activity at temperatures of 10 to 20°C, the activity decreases significantly as the temperature increases above 20°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 narrow optimum, about 20% of maximal activity at pH 7.0 and about 40% at pH 8.0, profile overview Pseudomonas sp.

General Information

General Information Comment Organism
metabolism the enzyme is important in the indole-3-acetic acid biosynthesis pathway together with the nitrilase, EC 3.5.5.1, which produces indole-3-amide, but also indole-3-acetic acid , overview Pseudomonas sp.