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Literature summary for 4.2.1.84 extracted from

  • Kobayashi, M.; Suzuki, T.; Fujita, T.; Masuda, M.; Shimizu, S.
    Occurrence of enzymes involved in biosynthesis of indole-3-acetic acid from indole-3-acetonitrile in plant-associated bacteria, Agrobacterium and Rhizobium (1995), Proc. Natl. Acad. Sci. USA, 92, 714-718.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0079
-
indole-3-acetonitrile pH 7.5, temperature not specified in the publication Agrobacterium tumefaciens
0.01
-
phenylacetonitrile pH 7.5, temperature not specified in the publication Agrobacterium tumefaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enzyme-bound Agrobacterium tumefaciens
Fe2+ enzyme-bound Agrobacterium tumefaciens
additional information the Agrobacterium tunefaciens enzyme does not contain Be, B, Mg, Al, Si, P, S, Ca, Ti, V, Cr, Mn, Ni, Cu, Zn, Se, Sr, Zr, Mo, Pd, Ag, Cd, Sn, Sb, Ba, Ta, W, Pt, Au, Hg, Pb, La, or Ce Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
4 * 27000, SDS-PAGE Agrobacterium tumefaciens
102000
-
gel filtration Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
indole-3-acetonitrile + H2O Sinorhizobium meliloti
-
(indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O Agrobacterium tumefaciens
-
(indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O Rhizobium leguminosarum
-
(indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O Mesorhizobium loti
-
(indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O Mesorhizobium loti 02-10055
-
(indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O Mesorhizobium loti 02-10056
-
(indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O Agrobacterium tumefaciens IAM B-261
-
(indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O Rhizobium leguminosarum 02-03119
-
(indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O Mesorhizobium loti 02-10101
-
(indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O Sinorhizobium meliloti 03-03046
-
(indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O Rhizobium leguminosarum 02-10230
-
(indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O Rhizobium leguminosarum 02-10041
-
(indol-3-yl)acetamide
-
?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens IAM B-261
-
-
-
Mesorhizobium loti
-
-
-
Mesorhizobium loti 02-10055
-
-
-
Mesorhizobium loti 02-10056
-
-
-
Mesorhizobium loti 02-10101
-
-
-
Rhizobium leguminosarum
-
-
-
Rhizobium leguminosarum 02-03119
-
-
-
Rhizobium leguminosarum 02-10041
-
-
-
Rhizobium leguminosarum 02-10230
-
-
-
Sinorhizobium meliloti
-
-
-
Sinorhizobium meliloti 03-03046
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.18
-
strain 02-10230, in absence of crotonamide, pH 7.5, temperature not specified in the publication Rhizobium leguminosarum
0.22
-
strain 03-03046, in absence of crotonamide, pH 7.5, temperature not specified in the publication Sinorhizobium meliloti
0.27
-
strain 02-10041, in absence of crotonamide, pH 7.5, temperature not specified in the publication Rhizobium leguminosarum
0.3
-
strain 03-03119, in absence of crotonamide, pH 7.5, temperature not specified in the publication Rhizobium leguminosarum
1.11
-
strain 02-10056, in presence of crotonamide, pH 7.5, temperature not specified in the publication Mesorhizobium loti
1.12
-
strain 02-10230, in presence of crotonamide, pH 7.5, temperature not specified in the publication Rhizobium leguminosarum
1.41
-
strain 02-10056, in absence of crotonamide, pH 7.5, temperature not specified in the publication Mesorhizobium loti
5.32
-
strain 02-10055, in presence of crotonamide, pH 7.5, temperature not specified in the publication Mesorhizobium loti
6.89
-
strain 02-10055, in absence of crotonamide, pH 7.5, temperature not specified in the publication Mesorhizobium loti
13.7
-
purified native enzyme, pH 7.5, temperature not specified in the publication Agrobacterium tumefaciens
116
-
strain 02-10101, in presence of crotonamide, pH 7.5, temperature not specified in the publication Mesorhizobium loti
168
-
strain 02-10101, in absence of crotonamide, pH 7.5, temperature not specified in the publication Mesorhizobium loti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
indole-3-acetonitrile + H2O
-
Sinorhizobium meliloti (indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O
-
Agrobacterium tumefaciens (indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O
-
Rhizobium leguminosarum (indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O
-
Mesorhizobium loti (indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O
-
Mesorhizobium loti 02-10055 (indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O
-
Mesorhizobium loti 02-10056 (indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O
-
Agrobacterium tumefaciens IAM B-261 (indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O
-
Rhizobium leguminosarum 02-03119 (indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O
-
Mesorhizobium loti 02-10101 (indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O
-
Sinorhizobium meliloti 03-03046 (indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O
-
Rhizobium leguminosarum 02-10230 (indol-3-yl)acetamide
-
?
indole-3-acetonitrile + H2O
-
Rhizobium leguminosarum 02-10041 (indol-3-yl)acetamide
-
?
additional information (indol-3-yl)acetamide is no substrate, no formation of iodoacetic acid Agrobacterium tumefaciens ?
-
?
additional information (indol-3-yl)acetamide is no substrate, no formation of iodoacetic acid Agrobacterium tumefaciens IAM B-261 ?
-
?
phenylacetonitrile + H2O 337% activity compared to indole-3-acetonitrile Sinorhizobium meliloti phenylacetamide
-
?
phenylacetonitrile + H2O 337% activity compared to indole-3-acetonitrile Agrobacterium tumefaciens phenylacetamide
-
?
phenylacetonitrile + H2O 337% activity compared to indole-3-acetonitrile Agrobacterium tumefaciens IAM B-261 phenylacetamide
-
?
phenylacetonitrile + H2O 337% activity compared to indole-3-acetonitrile Sinorhizobium meliloti 03-03046 phenylacetamide
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 27000, SDS-PAGE Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Sinorhizobium meliloti
7.5
-
assay at Agrobacterium tumefaciens
7.5
-
assay at Rhizobium leguminosarum
7.5
-
assay at Mesorhizobium loti

Expression

Organism Comment Expression
Rhizobium leguminosarum no activity in presence of crotonamide in strain 03-03119 down
Sinorhizobium meliloti no activity in strain 03-03046 in presence of crotonamide down
Rhizobium leguminosarum no activity in strain 02-10041 in absence of crotonamide up