KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0079 | - |
indole-3-acetonitrile | pH 7.5, temperature not specified in the publication | Agrobacterium tumefaciens | |
0.01 | - |
phenylacetonitrile | pH 7.5, temperature not specified in the publication | Agrobacterium tumefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | enzyme-bound | Agrobacterium tumefaciens | |
Fe2+ | enzyme-bound | Agrobacterium tumefaciens | |
additional information | the Agrobacterium tunefaciens enzyme does not contain Be, B, Mg, Al, Si, P, S, Ca, Ti, V, Cr, Mn, Ni, Cu, Zn, Se, Sr, Zr, Mo, Pd, Ag, Cd, Sn, Sb, Ba, Ta, W, Pt, Au, Hg, Pb, La, or Ce | Agrobacterium tumefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
4 * 27000, SDS-PAGE | Agrobacterium tumefaciens |
102000 | - |
gel filtration | Agrobacterium tumefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole-3-acetonitrile + H2O | Sinorhizobium meliloti | - |
(indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | Agrobacterium tumefaciens | - |
(indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | Rhizobium leguminosarum | - |
(indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | Mesorhizobium loti | - |
(indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | Mesorhizobium loti 02-10055 | - |
(indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | Mesorhizobium loti 02-10056 | - |
(indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | Agrobacterium tumefaciens IAM B-261 | - |
(indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | Rhizobium leguminosarum 02-03119 | - |
(indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | Mesorhizobium loti 02-10101 | - |
(indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | Sinorhizobium meliloti 03-03046 | - |
(indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | Rhizobium leguminosarum 02-10230 | - |
(indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | Rhizobium leguminosarum 02-10041 | - |
(indol-3-yl)acetamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | - |
- |
- |
Agrobacterium tumefaciens IAM B-261 | - |
- |
- |
Mesorhizobium loti | - |
- |
- |
Mesorhizobium loti 02-10055 | - |
- |
- |
Mesorhizobium loti 02-10056 | - |
- |
- |
Mesorhizobium loti 02-10101 | - |
- |
- |
Rhizobium leguminosarum | - |
- |
- |
Rhizobium leguminosarum 02-03119 | - |
- |
- |
Rhizobium leguminosarum 02-10041 | - |
- |
- |
Rhizobium leguminosarum 02-10230 | - |
- |
- |
Sinorhizobium meliloti | - |
- |
- |
Sinorhizobium meliloti 03-03046 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity | Agrobacterium tumefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.18 | - |
strain 02-10230, in absence of crotonamide, pH 7.5, temperature not specified in the publication | Rhizobium leguminosarum |
0.22 | - |
strain 03-03046, in absence of crotonamide, pH 7.5, temperature not specified in the publication | Sinorhizobium meliloti |
0.27 | - |
strain 02-10041, in absence of crotonamide, pH 7.5, temperature not specified in the publication | Rhizobium leguminosarum |
0.3 | - |
strain 03-03119, in absence of crotonamide, pH 7.5, temperature not specified in the publication | Rhizobium leguminosarum |
1.11 | - |
strain 02-10056, in presence of crotonamide, pH 7.5, temperature not specified in the publication | Mesorhizobium loti |
1.12 | - |
strain 02-10230, in presence of crotonamide, pH 7.5, temperature not specified in the publication | Rhizobium leguminosarum |
1.41 | - |
strain 02-10056, in absence of crotonamide, pH 7.5, temperature not specified in the publication | Mesorhizobium loti |
5.32 | - |
strain 02-10055, in presence of crotonamide, pH 7.5, temperature not specified in the publication | Mesorhizobium loti |
6.89 | - |
strain 02-10055, in absence of crotonamide, pH 7.5, temperature not specified in the publication | Mesorhizobium loti |
13.7 | - |
purified native enzyme, pH 7.5, temperature not specified in the publication | Agrobacterium tumefaciens |
116 | - |
strain 02-10101, in presence of crotonamide, pH 7.5, temperature not specified in the publication | Mesorhizobium loti |
168 | - |
strain 02-10101, in absence of crotonamide, pH 7.5, temperature not specified in the publication | Mesorhizobium loti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
indole-3-acetonitrile + H2O | - |
Sinorhizobium meliloti | (indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | - |
Agrobacterium tumefaciens | (indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | - |
Rhizobium leguminosarum | (indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | - |
Mesorhizobium loti | (indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | - |
Mesorhizobium loti 02-10055 | (indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | - |
Mesorhizobium loti 02-10056 | (indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | - |
Agrobacterium tumefaciens IAM B-261 | (indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | - |
Rhizobium leguminosarum 02-03119 | (indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | - |
Mesorhizobium loti 02-10101 | (indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | - |
Sinorhizobium meliloti 03-03046 | (indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | - |
Rhizobium leguminosarum 02-10230 | (indol-3-yl)acetamide | - |
? | |
indole-3-acetonitrile + H2O | - |
Rhizobium leguminosarum 02-10041 | (indol-3-yl)acetamide | - |
? | |
additional information | (indol-3-yl)acetamide is no substrate, no formation of iodoacetic acid | Agrobacterium tumefaciens | ? | - |
? | |
additional information | (indol-3-yl)acetamide is no substrate, no formation of iodoacetic acid | Agrobacterium tumefaciens IAM B-261 | ? | - |
? | |
phenylacetonitrile + H2O | 337% activity compared to indole-3-acetonitrile | Sinorhizobium meliloti | phenylacetamide | - |
? | |
phenylacetonitrile + H2O | 337% activity compared to indole-3-acetonitrile | Agrobacterium tumefaciens | phenylacetamide | - |
? | |
phenylacetonitrile + H2O | 337% activity compared to indole-3-acetonitrile | Agrobacterium tumefaciens IAM B-261 | phenylacetamide | - |
? | |
phenylacetonitrile + H2O | 337% activity compared to indole-3-acetonitrile | Sinorhizobium meliloti 03-03046 | phenylacetamide | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 27000, SDS-PAGE | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sinorhizobium meliloti |
7.5 | - |
assay at | Agrobacterium tumefaciens |
7.5 | - |
assay at | Rhizobium leguminosarum |
7.5 | - |
assay at | Mesorhizobium loti |
Organism | Comment | Expression |
---|---|---|
Rhizobium leguminosarum | no activity in presence of crotonamide in strain 03-03119 | down |
Sinorhizobium meliloti | no activity in strain 03-03046 in presence of crotonamide | down |
Rhizobium leguminosarum | no activity in strain 02-10041 in absence of crotonamide | up |