Application | Comment | Organism |
---|---|---|
synthesis | transformation of benzonitrile into benzohydroxamic acid performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Klebsiella oxytoca strain 38.1.2, the second step is a cellfree extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | Klebsiella oxytoca |
synthesis | transformation of benzonitrile into benzohydroxamic acid performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Raoultella terrigena srain 77.1, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | Raoultella terrigena |
synthesis | transformation of benzonitrile into benzohydroxamic acid performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from Rhodococcus erythropolis A4 containing nitrile hydratase, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-3-butenenitrile + H2O | Raoultella terrigena | - |
? | - |
? | |
2-methyl-3-butenenitrile + H2O | Klebsiella oxytoca | - |
? | - |
? | |
3-chlorobenzonitrile + H2O | Klebsiella oxytoca | - |
3-chlorobenzamide | - |
? | |
3-hydroxybenzonitrile + H2O | Raoultella terrigena | - |
3-hydroxybenzamide | - |
? | |
3-hydroxybenzonitrile + H2O | Klebsiella oxytoca | - |
3-hydroxybenzamide | - |
? | |
3-tolunitrile + H2O | Raoultella terrigena | - |
? | - |
? | |
3-tolunitrile + H2O | Klebsiella oxytoca | - |
? | - |
? | |
4-aminobenzonitrile + H2O | Raoultella terrigena | - |
4-aminobenzamide | - |
? | |
4-aminobenzonitrile + H2O | Klebsiella oxytoca | - |
4-aminobenzamide | - |
? | |
4-chlorobenzonitrile + H2O | Raoultella terrigena | - |
4-chlorobenzamide | - |
? | |
4-chlorobenzonitrile + H2O | Klebsiella oxytoca | - |
4-chlorobenzamide | - |
? | |
benzonitrile + H2O | Klebsiella oxytoca | performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Klebsiella oxytoca strain 38.1.2, the second step is cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | benzamide | - |
? | |
benzonitrile + H2O | Raoultella terrigena | performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Raoultella terrigena srain 77.1, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | benzamide | - |
? | |
benzonitrile + H2O | Klebsiella oxytoca 38.1.2 | performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Klebsiella oxytoca strain 38.1.2, the second step is cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | benzamide | - |
? | |
benzonitrile + H2O | Raoultella terrigena 77.1 | performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Raoultella terrigena srain 77.1, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | benzamide | - |
? | |
benzonitrile + hydroxylamine + H2O | Rhodococcus erythropolis | performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from Rhodococcus erythropolis A4 containing nitrile hydratase, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | benzohydroxamic acid + NH3 | - |
? | |
benzonitrile + hydroxylamine + H2O | Rhodococcus erythropolis A4 | performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from Rhodococcus erythropolis A4 containing nitrile hydratase, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | benzohydroxamic acid + NH3 | - |
? | |
butyronitrile + H2O | Raoultella terrigena | - |
butyramide | - |
? | |
butyronitrile + H2O | Klebsiella oxytoca | - |
butyramide | - |
? | |
butyronitrile + H2O | Klebsiella oxytoca 38.1.2 | - |
butyramide | - |
? | |
butyronitrile + H2O | Raoultella terrigena 77.1 | - |
butyramide | - |
? | |
additional information | Klebsiella oxytoca | substrate specificity of strain 38.1.2, no activity with 4-tolunitrile, overview | ? | - |
? | |
additional information | Raoultella terrigena | substrate specificity of strain 77.1, no activity with 4-tolunitrile and 3-chlorobenzonitrile, overview | ? | - |
? | |
additional information | Klebsiella oxytoca 38.1.2 | substrate specificity of strain 38.1.2, no activity with 4-tolunitrile, overview | ? | - |
? | |
additional information | Raoultella terrigena 77.1 | substrate specificity of strain 77.1, no activity with 4-tolunitrile and 3-chlorobenzonitrile, overview | ? | - |
? | |
propionitrile + H2O | Raoultella terrigena | - |
propionamide | - |
? | |
propionitrile + H2O | Klebsiella oxytoca | - |
propionamide | - |
? | |
propionitrile + H2O | Klebsiella oxytoca 38.1.2 | - |
propionamide | - |
? | |
propionitrile + H2O | Raoultella terrigena 77.1 | - |
propionamide | - |
? | |
valeronitrile + H2O | Raoultella terrigena | - |
valeramide | - |
? | |
valeronitrile + H2O | Klebsiella oxytoca | - |
valeramide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella oxytoca | - |
- |
- |
Klebsiella oxytoca 38.1.2 | - |
- |
- |
Raoultella terrigena | - |
- |
- |
Raoultella terrigena 77.1 | - |
- |
- |
Rhodococcus erythropolis | - |
- |
- |
Rhodococcus erythropolis A4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-3-butenenitrile + H2O | - |
Raoultella terrigena | ? | - |
? | |
2-methyl-3-butenenitrile + H2O | - |
Klebsiella oxytoca | ? | - |
? | |
3-chlorobenzonitrile + H2O | - |
Klebsiella oxytoca | 3-chlorobenzamide | - |
? | |
3-hydroxybenzonitrile + H2O | - |
Raoultella terrigena | 3-hydroxybenzamide | - |
? | |
3-hydroxybenzonitrile + H2O | - |
Klebsiella oxytoca | 3-hydroxybenzamide | - |
? | |
3-tolunitrile + H2O | - |
Raoultella terrigena | ? | - |
? | |
3-tolunitrile + H2O | - |
Klebsiella oxytoca | ? | - |
? | |
4-aminobenzonitrile + H2O | - |
Raoultella terrigena | 4-aminobenzamide | - |
? | |
4-aminobenzonitrile + H2O | - |
Klebsiella oxytoca | 4-aminobenzamide | - |
? | |
4-chlorobenzonitrile + H2O | - |
Raoultella terrigena | 4-chlorobenzamide | - |
? | |
4-chlorobenzonitrile + H2O | - |
Klebsiella oxytoca | 4-chlorobenzamide | - |
? | |
benzonitrile + H2O | - |
Raoultella terrigena | benzamide | - |
? | |
benzonitrile + H2O | - |
Klebsiella oxytoca | benzamide | - |
? | |
benzonitrile + H2O | performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Klebsiella oxytoca strain 38.1.2, the second step is cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | Klebsiella oxytoca | benzamide | - |
? | |
benzonitrile + H2O | performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Raoultella terrigena srain 77.1, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | Raoultella terrigena | benzamide | - |
? | |
benzonitrile + H2O | - |
Klebsiella oxytoca 38.1.2 | benzamide | - |
? | |
benzonitrile + H2O | performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Klebsiella oxytoca strain 38.1.2, the second step is cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | Klebsiella oxytoca 38.1.2 | benzamide | - |
? | |
benzonitrile + H2O | - |
Raoultella terrigena 77.1 | benzamide | - |
? | |
benzonitrile + H2O | performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from recombinant Escherichia coli strain expressing nitrile hydratase from Raoultella terrigena srain 77.1, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | Raoultella terrigena 77.1 | benzamide | - |
? | |
benzonitrile + hydroxylamine + H2O | - |
Rhodococcus erythropolis | benzohydroxamic acid + NH3 | - |
? | |
benzonitrile + hydroxylamine + H2O | performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from Rhodococcus erythropolis A4 containing nitrile hydratase, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | Rhodococcus erythropolis | benzohydroxamic acid + NH3 | - |
? | |
benzonitrile + hydroxylamine + H2O | - |
Rhodococcus erythropolis A4 | benzohydroxamic acid + NH3 | - |
? | |
benzonitrile + hydroxylamine + H2O | performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step is catalyzed by a cell-free extract from Rhodococcus erythropolis A4 containing nitrile hydratase, the second step is a cell-free extract from Rhodococcus erythropolis A4 amidase, EC 3.5.1.4 | Rhodococcus erythropolis A4 | benzohydroxamic acid + NH3 | - |
? | |
butyronitrile + H2O | - |
Raoultella terrigena | butyramide | - |
? | |
butyronitrile + H2O | - |
Klebsiella oxytoca | butyramide | - |
? | |
butyronitrile + H2O | - |
Klebsiella oxytoca 38.1.2 | butyramide | - |
? | |
butyronitrile + H2O | - |
Raoultella terrigena 77.1 | butyramide | - |
? | |
additional information | substrate specificity of strain 38.1.2, no activity with 4-tolunitrile, overview | Klebsiella oxytoca | ? | - |
? | |
additional information | substrate specificity of strain 77.1, no activity with 4-tolunitrile and 3-chlorobenzonitrile, overview | Raoultella terrigena | ? | - |
? | |
additional information | substrate specificity of strain 38.1.2, no activity with 4-tolunitrile, overview | Klebsiella oxytoca 38.1.2 | ? | - |
? | |
additional information | substrate specificity of strain 77.1, no activity with 4-tolunitrile and 3-chlorobenzonitrile, overview | Raoultella terrigena 77.1 | ? | - |
? | |
propionitrile + H2O | - |
Raoultella terrigena | propionamide | - |
? | |
propionitrile + H2O | - |
Klebsiella oxytoca | propionamide | - |
? | |
propionitrile + H2O | - |
Klebsiella oxytoca 38.1.2 | propionamide | - |
? | |
propionitrile + H2O | - |
Raoultella terrigena 77.1 | propionamide | - |
? | |
valeronitrile + H2O | - |
Raoultella terrigena | valeramide | - |
? | |
valeronitrile + H2O | - |
Klebsiella oxytoca | valeramide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Rhodococcus erythropolis |
28 | - |
assay at | Raoultella terrigena |
28 | - |
assay at | Klebsiella oxytoca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rhodococcus erythropolis |
8 | - |
assay at | Raoultella terrigena |
8 | - |
assay at | Klebsiella oxytoca |