Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cobalt | belongs to the cobalt NHase family of enzymes | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | - |
- |
- |
Serratia marcescens ZJB-09104 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-cyanopyridine + H2O | - |
Serratia marcescens | pyridine-2-carbamide | - |
? | |
2-cyanopyridine + H2O | - |
Serratia marcescens ZJB-09104 | pyridine-2-carbamide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NHase | - |
Serratia marcescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Serratia marcescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 65 | the enzyme activity gradually reduces with the further increase of temperature from 50 to 65°C, the thermostability of NHase is comparatively good under 50°C, while the enzyme activity is obviously lost when the cells are preincubated over 50°C | Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
the optimum nitrile hydratase activity is observed in the Tris-HCl buffer at a pH of 8.0 | Serratia marcescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.3 | 10.4 | at pH value of 4.3 and 10.4 enzyme activities are reduced to 24 and 25% of the maximum activity, respectively | Serratia marcescens |