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Literature summary for 4.2.1.84 extracted from

  • Prasad, S.; Raj, J.; Bhalla, T.
    Purification of a hyperactive nitrile hydratase from resting cells of Rhodococcus rhodochrous PA-34 (2009), Indian J. Microbiol., 49, 237-242.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.84

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 complete inhibition at 1 mM Rhodococcus rhodochrous
ammonium persulfate 4.5% inhibition at 1 mM Rhodococcus rhodochrous
Ca2+ 10% inhibition at 1 mM Rhodococcus rhodochrous
Cd2+ 8% inhibition at 1 mM Rhodococcus rhodochrous
Co2+ 2% inhibition at 1 mM Rhodococcus rhodochrous
Cu2+ 7% inhibition at 1 mM Rhodococcus rhodochrous
EDTA 12.1% inhibition at 1 mM Rhodococcus rhodochrous
Fe2+ 5% inhibition at 1 mM Rhodococcus rhodochrous
Hg+ 89.3% inhibition at 1 mM Rhodococcus rhodochrous
hydroxylamine 62.6% inhibition at 1 mM Rhodococcus rhodochrous
iodoacetic acid 65% inhibition at 1 mM Rhodococcus rhodochrous
L-ascorbic acid 54.6% inhibition at 1 mM Rhodococcus rhodochrous
Mg2+ 4% inhibition at 1 mM Rhodococcus rhodochrous
Mn2+ 8% inhibition at 1 mM Rhodococcus rhodochrous
additional information not inhibited by dithiothreitol, phenylmethylsulfonylfluoride, and beta-mercaptoethanol Rhodococcus rhodochrous
Pb2+ 94.5% inhibition at 1 mM Rhodococcus rhodochrous
phenyl hydrazine 68% inhibition at 1 mM Rhodococcus rhodochrous
Sodium azide 26.6% inhibition at 1 mM Rhodococcus rhodochrous
Urea 13.9% inhibition at 1 mM Rhodococcus rhodochrous

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
167
-
 3-Cyanopyridine in 0.1 M potassium phosphate buffer, pH 8.0, at 40°C Rhodococcus rhodochrous

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25040
-
 1 * 25040 + 1 * 30600, estimated from SDS-PAGE Rhodococcus rhodochrous
30600
-
 1 * 25040 + 1 * 30600, estimated from SDS-PAGE Rhodococcus rhodochrous
86000
-
 holoenzyme, native PAGE Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
-
-
Rhodococcus rhodochrous PA-34
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, Sephacryl S 300 gel filtration, and DEAE column chromatography Rhodococcus rhodochrous

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.16
-
 cell-free extract, at pH 8.0, at 40°C Rhodococcus rhodochrous
46.83
-
 after 11.3fold purification, at pH 8.0, at 40°C Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-cyanopyridine + H2O
-
 Rhodococcus rhodochrous pyridine-3-carbamide
-
 ?
3-cyanopyridine + H2O
-
 Rhodococcus rhodochrous PA-34 pyridine-3-carbamide
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 25040 + 1 * 30600, estimated from SDS-PAGE Rhodococcus rhodochrous

Synonyms

Synonyms Comment Organism
NHase
-
 Rhodococcus rhodochrous

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Rhodococcus rhodochrous

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 half-life is 2 h at 40°C and 0.5 h at 50°C, below and above 40°C NHase activity decreases drastically Rhodococcus rhodochrous

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Rhodococcus rhodochrous

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5
-
 Rhodococcus rhodochrous

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 the pH below 5 and above 10 drastically decreases the activity of purified NHase Rhodococcus rhodochrous