Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.84 extracted from

  • Ma, Y.; Yu, H.; Pan, W.; Liu, C.; Zhang, S.; Shen, Z.
    Identification of nitrile hydratase-producing Rhodococcus ruber TH and characterization of an amiE-negative mutant (2010), Biores. Technol., 101, 285-291.
    View publication on PubMed
Search for more literature for 4.2.1.84

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhodococcus ruber

Protein Variants

Protein Variants Comment Organism
additional information construction of an amidase-negative, amiE-, recombinant strain TH3 with 25% increased nitrile hydratase activity and 60% reduced amidase activity compared to the wild-type strain TH. Usage of TH3 free cells as biocatalysts at 18°C for acrylamide production, increased by 23% and 87% reduced acrylic acid by-product formation conmpared to the wild-type Rhodococcus ruber

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acrylonitrile + H2O Rhodococcus ruber
-
 acrylamide
-
 ?
acrylonitrile + H2O Rhodococcus ruber TH
-
 acrylamide
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus ruber
-
-
-
Rhodococcus ruber TH
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylonitrile + H2O
-
 Rhodococcus ruber acrylamide
-
 ?
acrylonitrile + H2O
-
 Rhodococcus ruber TH acrylamide
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at, in vivo in cells Rhodococcus ruber

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at, in vivo in cells Rhodococcus ruber

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone
-
 Rhodococcus ruber