Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2S2O8 | 23% inhibition at 10 mM, 22% inhibition at 1 mM | Mesorhizobium sp. | |
Ag2SO3 | complete inhibition at 1 mM | Mesorhizobium sp. | |
CuSO4 | 62% inhibition at 10 mM, 35% inhibition at 1 mM | Mesorhizobium sp. | |
EDTA | 64% inhibition at 10 mM, 22% inhibition at 1 mM | Mesorhizobium sp. | |
Fe2+ | 22% inhibition at 10 mM, 11% inhibition at 1 mM | Mesorhizobium sp. | |
H2O2 | 89% inhibition at 10 mM, 29% inhibition at 1 mM | Mesorhizobium sp. | |
Mg2+ | 59% inhibition at 10 mM, 16% inhibition at 1 mM | Mesorhizobium sp. | |
additional information | no inhibition by 2-mercaptoethanol | Mesorhizobium sp. | |
Sodium azide | 74% inhibition at 10 mM, 22% inhibition at 1 mM | Mesorhizobium sp. | |
Zn2+ | 71% inhibition at 10 mM, 54% inhibition at 1 mM | Mesorhizobium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
acrylonitrile | pH 7.5, 37°C | Mesorhizobium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co3+ | a cobalt-containing NHase in strain F28 | Mesorhizobium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylonitrile + H2O | Mesorhizobium sp. | - |
acrylamide | - |
? | |
benzonitrile + H2O | Mesorhizobium sp. | - |
benzoic acid amide | - |
? | |
isobutyronitrile + H2O | Mesorhizobium sp. | - |
isobutyric acid amide | - |
? | |
isobutyronitrile + H2O | Mesorhizobium sp. BNC1 | - |
isobutyric acid amide | - |
? | |
propionitrile + H2O | Mesorhizobium sp. | - |
propionic acid amide | - |
? | |
propionitrile + H2O | Mesorhizobium sp. BNC1 | - |
propionic acid amide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesorhizobium sp. | - |
strain F28, isolated from a wastewater treatment system in a polyacrylonitrile fibre factory | - |
Mesorhizobium sp. BNC1 | - |
strain F28, isolated from a wastewater treatment system in a polyacrylonitrile fibre factory | - |
Purification (Comment) | Organism |
---|---|
native enzyme from strain F28 5fold by ammonium sulfate fractionation, anion exchange chromatography and gel filtration | Mesorhizobium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimum growth temperature of strain F28 is 37°C | Mesorhizobium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
192 | - |
strain F28, substrate acrylonitrile | Mesorhizobium sp. |
686 | - |
purified native enzyme from strain F28, substrate acrylonitrile | Mesorhizobium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrylonitrile + H2O | - |
Mesorhizobium sp. | acrylamide | - |
? | |
acrylonitrile + H2O | best substrate | Mesorhizobium sp. | acrylamide | - |
? | |
benzonitrile + H2O | - |
Mesorhizobium sp. | benzoic acid amide | - |
? | |
benzonitrile + H2O | 15% of the activity with acrylonitrile | Mesorhizobium sp. | benzoic acid amide | - |
? | |
isobutyronitrile + H2O | - |
Mesorhizobium sp. | isobutyric acid amide | - |
? | |
isobutyronitrile + H2O | 71% of the activity with acrylonitrile | Mesorhizobium sp. | isobutyric acid amide | - |
? | |
isobutyronitrile + H2O | - |
Mesorhizobium sp. BNC1 | isobutyric acid amide | - |
? | |
isobutyronitrile + H2O | 71% of the activity with acrylonitrile | Mesorhizobium sp. BNC1 | isobutyric acid amide | - |
? | |
additional information | the NHase active site of the strain F28 might consist of cysteine and serine | Mesorhizobium sp. | ? | - |
? | |
additional information | the NHase active site of the strain F28 might consist of cysteine and serine | Mesorhizobium sp. BNC1 | ? | - |
? | |
propionitrile + H2O | - |
Mesorhizobium sp. | propionic acid amide | - |
? | |
propionitrile + H2O | 17% of the activity with acrylonitrile | Mesorhizobium sp. | propionic acid amide | - |
? | |
propionitrile + H2O | - |
Mesorhizobium sp. BNC1 | propionic acid amide | - |
? | |
propionitrile + H2O | 17% of the activity with acrylonitrile | Mesorhizobium sp. BNC1 | propionic acid amide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NHase | - |
Mesorhizobium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | - |
Mesorhizobium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | at 30°C and 20°C the enzyme activity decreases by 13% and 26% of the maximal activity, respectively, the enzyme is inactive at 55-65°C, high activity at 45°C | Mesorhizobium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
purified enzyme, stable up to | Mesorhizobium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Mesorhizobium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | the enzyme retains over 50% activity at pH 5.0 and pH 8.8, but loses activity below pH 4.0 or above pH 9.0 | Mesorhizobium sp. |