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Literature summary for 4.2.1.84 extracted from

  • Feng, Y.; Chen, P.; Wen, F.; Hsiao, W.; Lee, C.
    Nitrile hydratase from Mesorhizobium sp. F28 and its potential for nitrile biotransformation (2008), Process Biochem., 43, 1391-1397.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2S2O8 23% inhibition at 10 mM, 22% inhibition at 1 mM Mesorhizobium sp.
Ag2SO3 complete inhibition at 1 mM Mesorhizobium sp.
CuSO4 62% inhibition at 10 mM, 35% inhibition at 1 mM Mesorhizobium sp.
EDTA 64% inhibition at 10 mM, 22% inhibition at 1 mM Mesorhizobium sp.
Fe2+ 22% inhibition at 10 mM, 11% inhibition at 1 mM Mesorhizobium sp.
H2O2 89% inhibition at 10 mM, 29% inhibition at 1 mM Mesorhizobium sp.
Mg2+ 59% inhibition at 10 mM, 16% inhibition at 1 mM Mesorhizobium sp.
additional information no inhibition by 2-mercaptoethanol Mesorhizobium sp.
Sodium azide 74% inhibition at 10 mM, 22% inhibition at 1 mM Mesorhizobium sp.
Zn2+ 71% inhibition at 10 mM, 54% inhibition at 1 mM Mesorhizobium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
acrylonitrile pH 7.5, 37°C Mesorhizobium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co3+ a cobalt-containing NHase in strain F28 Mesorhizobium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acrylonitrile + H2O Mesorhizobium sp.
-
acrylamide
-
?
benzonitrile + H2O Mesorhizobium sp.
-
benzoic acid amide
-
?
isobutyronitrile + H2O Mesorhizobium sp.
-
isobutyric acid amide
-
?
isobutyronitrile + H2O Mesorhizobium sp. BNC1
-
isobutyric acid amide
-
?
propionitrile + H2O Mesorhizobium sp.
-
propionic acid amide
-
?
propionitrile + H2O Mesorhizobium sp. BNC1
-
propionic acid amide
-
?

Organism

Organism UniProt Comment Textmining
Mesorhizobium sp.
-
strain F28, isolated from a wastewater treatment system in a polyacrylonitrile fibre factory
-
Mesorhizobium sp. BNC1
-
strain F28, isolated from a wastewater treatment system in a polyacrylonitrile fibre factory
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain F28 5fold by ammonium sulfate fractionation, anion exchange chromatography and gel filtration Mesorhizobium sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimum growth temperature of strain F28 is 37°C Mesorhizobium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
192
-
strain F28, substrate acrylonitrile Mesorhizobium sp.
686
-
purified native enzyme from strain F28, substrate acrylonitrile Mesorhizobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylonitrile + H2O
-
Mesorhizobium sp. acrylamide
-
?
acrylonitrile + H2O best substrate Mesorhizobium sp. acrylamide
-
?
benzonitrile + H2O
-
Mesorhizobium sp. benzoic acid amide
-
?
benzonitrile + H2O 15% of the activity with acrylonitrile Mesorhizobium sp. benzoic acid amide
-
?
isobutyronitrile + H2O
-
Mesorhizobium sp. isobutyric acid amide
-
?
isobutyronitrile + H2O 71% of the activity with acrylonitrile Mesorhizobium sp. isobutyric acid amide
-
?
isobutyronitrile + H2O
-
Mesorhizobium sp. BNC1 isobutyric acid amide
-
?
isobutyronitrile + H2O 71% of the activity with acrylonitrile Mesorhizobium sp. BNC1 isobutyric acid amide
-
?
additional information the NHase active site of the strain F28 might consist of cysteine and serine Mesorhizobium sp. ?
-
?
additional information the NHase active site of the strain F28 might consist of cysteine and serine Mesorhizobium sp. BNC1 ?
-
?
propionitrile + H2O
-
Mesorhizobium sp. propionic acid amide
-
?
propionitrile + H2O 17% of the activity with acrylonitrile Mesorhizobium sp. propionic acid amide
-
?
propionitrile + H2O
-
Mesorhizobium sp. BNC1 propionic acid amide
-
?
propionitrile + H2O 17% of the activity with acrylonitrile Mesorhizobium sp. BNC1 propionic acid amide
-
?

Synonyms

Synonyms Comment Organism
NHase
-
Mesorhizobium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 45
-
Mesorhizobium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 at 30°C and 20°C the enzyme activity decreases by 13% and 26% of the maximal activity, respectively, the enzyme is inactive at 55-65°C, high activity at 45°C Mesorhizobium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
purified enzyme, stable up to Mesorhizobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Mesorhizobium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 the enzyme retains over 50% activity at pH 5.0 and pH 8.8, but loses activity below pH 4.0 or above pH 9.0 Mesorhizobium sp.