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Literature summary for 4.2.1.84 extracted from

  • Liu, J.; Yu, H.; Shen, Z.
    Insights into thermal stability of thermophilic nitrile hydratases by molecular dynamics simulation (2008), J. Mol. Graph. Model., 27, 529-535.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information improvement of thermal stability of the industrialized mesophilic NHase by introducing stable salt-bridge interactions into its thermal sensitive regions Bacillus sp. (in: Bacteria)
additional information improvement of thermal stability of the industrialized mesophilic NHase by introducing stable salt-bridge interactions into its thermal sensitive regions Pseudonocardia thermophila

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
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-
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Bacillus sp. (in: Bacteria) SC-105-1
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-
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Pseudonocardia thermophila Q7SID2 cobalt-containing subunit alpha; strain JCM3095
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Pseudonocardia thermophila JCM 3095 Q7SID2 cobalt-containing subunit alpha; strain JCM3095
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Reaction

Reaction Comment Organism Reaction ID
an aliphatic amide = a nitrile + H2O thermal-stable mechanism of thermophilic nitrile hydratases, molecular dynamic simulation, overview Bacillus sp. (in: Bacteria)
an aliphatic amide = a nitrile + H2O thermal-stable mechanism of thermophilic nitrile hydratases, molecular dynamic simulation, overview Pseudonocardia thermophila

Subunits

Subunits Comment Organism
More structure analysis, overview Pseudonocardia thermophila
More structure analysis and salt-bridge interactions involving main chain atoms in region A1 of 1V29, overview Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
NHase
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Bacillus sp. (in: Bacteria)
NHase
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Pseudonocardia thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
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determination of thermal stability of thermophilic nitrile hydratases by molecular dynamics simulation using crystal structures with PDB ID 1UGQ. In 1UGQ, the absence of a charged residue decreases the thermal sensitivity of region B1, and the formation of a small beta-sheet containing a stable salt-bridge in C-beta-terminal significantly enhances the thermal stability Pseudonocardia thermophila
additional information
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determination of thermal stability of thermophilic nitrile hydratases by molecular dynamics simulation using crystal structures with PDB ID 1V29. In 1V29, region A1 is stabilized by a wellorganized hook-hook like cluster with multiple salt-bridge interactions, region A2 is stabilized by two strong salt-bridge interactions of Glu52-Arg332 and Glu334-Arg332 Bacillus sp. (in: Bacteria)