Protein Variants | Comment | Organism |
---|---|---|
additional information | improvement of thermal stability of the industrialized mesophilic NHase by introducing stable salt-bridge interactions into its thermal sensitive regions | Bacillus sp. (in: Bacteria) |
additional information | improvement of thermal stability of the industrialized mesophilic NHase by introducing stable salt-bridge interactions into its thermal sensitive regions | Pseudonocardia thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) SC-105-1 | - |
- |
- |
Pseudonocardia thermophila | Q7SID2 | cobalt-containing subunit alpha; strain JCM3095 | - |
Pseudonocardia thermophila JCM 3095 | Q7SID2 | cobalt-containing subunit alpha; strain JCM3095 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aliphatic amide = a nitrile + H2O | thermal-stable mechanism of thermophilic nitrile hydratases, molecular dynamic simulation, overview | Bacillus sp. (in: Bacteria) | |
an aliphatic amide = a nitrile + H2O | thermal-stable mechanism of thermophilic nitrile hydratases, molecular dynamic simulation, overview | Pseudonocardia thermophila |
Subunits | Comment | Organism |
---|---|---|
More | structure analysis, overview | Pseudonocardia thermophila |
More | structure analysis and salt-bridge interactions involving main chain atoms in region A1 of 1V29, overview | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
NHase | - |
Bacillus sp. (in: Bacteria) |
NHase | - |
Pseudonocardia thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
determination of thermal stability of thermophilic nitrile hydratases by molecular dynamics simulation using crystal structures with PDB ID 1UGQ. In 1UGQ, the absence of a charged residue decreases the thermal sensitivity of region B1, and the formation of a small beta-sheet containing a stable salt-bridge in C-beta-terminal significantly enhances the thermal stability | Pseudonocardia thermophila |
additional information | - |
determination of thermal stability of thermophilic nitrile hydratases by molecular dynamics simulation using crystal structures with PDB ID 1V29. In 1V29, region A1 is stabilized by a wellorganized hook-hook like cluster with multiple salt-bridge interactions, region A2 is stabilized by two strong salt-bridge interactions of Glu52-Arg332 and Glu334-Arg332 | Bacillus sp. (in: Bacteria) |