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Literature summary for 4.2.1.84 extracted from

  • Yano, T.; Ozawa, T.; Masuda, H.
    Structural and functional model systems for analysis of the active center of nitrile hydratase (2008), Chem. Lett., 37, 672-677.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information enzyme-ligand complex kinetic constants, overview Pseudonocardia thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Co3+ the unique active site structure of metalloenzyme nitrile hydratase includes a central metal ion, Co3+ or Fe3+, coordinated octahedrally by two amide nitrogens from the peptide backbone, one cysteine sulfur and two oxidized cysteine sulfurs, Cys-SO and Cys-SO2 Pseudonocardia thermophila
Fe3+ the unique active site structure of metalloenzyme nitrile hydratase includes a central metal ion, Co3+ or Fe3+, coordinated octahedrally by two amide nitrogens from the peptide backbone, one cysteine sulfur and two oxidized cysteine sulfurs, Cys-SO and Cys-SO2 Pseudonocardia thermophila
additional information redox potentials and structures of metal ion-enzyme complexes, overview Pseudonocardia thermophila

Organism

Organism UniProt Comment Textmining
Pseudonocardia thermophila
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-
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Pseudonocardia thermophila JCM 3095
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-
-

Reaction

Reaction Comment Organism Reaction ID
an aliphatic amide = a nitrile + H2O reaction mechanism, overview Pseudonocardia thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NHase hydrates a nitrile to provide the corresponding amide product via an addition reaction of one water molecule, active site structure involving amidate nitrogen donors from the peptide backbone, overview Pseudonocardia thermophila ?
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?
additional information NHase hydrates a nitrile to provide the corresponding amide product via an addition reaction of one water molecule, active site structure involving amidate nitrogen donors from the peptide backbone, overview Pseudonocardia thermophila JCM 3095 ?
-
?
tert-butylisonitrile + H2O
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Pseudonocardia thermophila tert-butyl amide
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?
tert-butylisonitrile + H2O
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Pseudonocardia thermophila JCM 3095 tert-butyl amide
-
?

Synonyms

Synonyms Comment Organism
NHase
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Pseudonocardia thermophila