KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | enzyme-ligand complex kinetic constants, overview | Pseudonocardia thermophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co3+ | the unique active site structure of metalloenzyme nitrile hydratase includes a central metal ion, Co3+ or Fe3+, coordinated octahedrally by two amide nitrogens from the peptide backbone, one cysteine sulfur and two oxidized cysteine sulfurs, Cys-SO and Cys-SO2 | Pseudonocardia thermophila | |
Fe3+ | the unique active site structure of metalloenzyme nitrile hydratase includes a central metal ion, Co3+ or Fe3+, coordinated octahedrally by two amide nitrogens from the peptide backbone, one cysteine sulfur and two oxidized cysteine sulfurs, Cys-SO and Cys-SO2 | Pseudonocardia thermophila | |
additional information | redox potentials and structures of metal ion-enzyme complexes, overview | Pseudonocardia thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudonocardia thermophila | - |
- |
- |
Pseudonocardia thermophila JCM 3095 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aliphatic amide = a nitrile + H2O | reaction mechanism, overview | Pseudonocardia thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NHase hydrates a nitrile to provide the corresponding amide product via an addition reaction of one water molecule, active site structure involving amidate nitrogen donors from the peptide backbone, overview | Pseudonocardia thermophila | ? | - |
? | |
additional information | NHase hydrates a nitrile to provide the corresponding amide product via an addition reaction of one water molecule, active site structure involving amidate nitrogen donors from the peptide backbone, overview | Pseudonocardia thermophila JCM 3095 | ? | - |
? | |
tert-butylisonitrile + H2O | - |
Pseudonocardia thermophila | tert-butyl amide | - |
? | |
tert-butylisonitrile + H2O | - |
Pseudonocardia thermophila JCM 3095 | tert-butyl amide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NHase | - |
Pseudonocardia thermophila |