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Literature summary for 4.2.1.84 extracted from

  • Song, L.; Yuan, H.J.; Coffey, L.; Doran, J.; Wang, M.X.; Qian, S.; OReilly, C.
    Efficient expression in E. coli of an enantioselective nitrile hydratase from Rhodococcus erythropolis (2007), Biotechnol. Lett., 30, 755-762.
    View publication on PubMed
Search for more literature for 4.2.1.84

Cloned(Commentary)

Cloned (Comment) Organism
co-expression of recombinant strains YHJ-1 to YHJ-4 with plasmids encoding the chaperons pG-KJE8, pGro7, pKJE7, pG-Tf2 and pTf16 Rhodococcus erythropolis
expression in Escherichia coli, NovaBlue, series of recombinant plasmids with genes encoding NHase (nha1: alpha subunit and nha2: beta subunit) and P44k protein (nha3), different positions and combinations Rhodococcus erythropolis
YHJ-1, Escherichia coli strain BL21(DE3) with plasmid pYHJ1 (alphabeta+P44k) Rhodococcus erythropolis
YHJ-2, Escherichia coli strain BL21(DE3) with plasmid pYHJ2 (alpha+betaP44k) Rhodococcus erythropolis
YHJ-3, Escherichia coli strain BL21(DE3) with plasmid pYHJ3 (alphabeta) Rhodococcus erythropolis
YHJ-4, Escherichia coli strain BL21(DE3) with plasmid pYHJ4 (alpha+beta) Rhodococcus erythropolis
YHJ-5, Escherichia coli strain BL21(DE3) co-expressing plasmids pYHJ3/pYHJ5 (P44k) Rhodococcus erythropolis
YHJ-6, Escherichia coli strain BL21(DE3) co-expressing plasmids pYHJ4/pYHJ5 Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
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 origin of genes encoding NHase (nha1 and nha2) and P44k protein (nha3)
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Rhodococcus erythropolis AJ270
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 origin of genes encoding NHase (nha1 and nha2) and P44k protein (nha3)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 0.18 +/- 0.009, micromol/min/ml, maximum activity after 21 h, strain YHJ-5 Rhodococcus erythropolis
additional information
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 0.3 +/- 0.01, micromol/min/ml, maximum activity after 21 h, strain YHJ-2 Rhodococcus erythropolis
additional information
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 1.12 +/- 0.03, micromol/min/ml, maximum activity after 21 h, strain YHJ-3 Rhodococcus erythropolis
additional information
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 4.96 +/- 0.18, micromol/min/ml, maximum activity after 6 h, strain YHJ-1 Rhodococcus erythropolis
additional information
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 chaperone protein DnaK-DnaJ-GrpE (pKJE7) lead to 20% decrease of normal NHase activity in the strains YHJ-1 and YHJ-3 Rhodococcus erythropolis
additional information
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 chaperone proteins lead to loss of NHase activity in the strain YHJ-2 Rhodococcus erythropolis
additional information
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 GroES/GroEL chaperone leads to doubling of NHase activity in the strain YHJ-3 Rhodococcus erythropolis
additional information
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 no detectable effect of chaperone proteins on NHase activity in the strain YHJ-4 Rhodococcus erythropolis
additional information
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 no detectable effect of GroES/GroEL chaperone on NHase activity in the strain YHJ-1 Rhodococcus erythropolis
additional information
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 no detectable NHase activity in the strains YHJ-4 and YHJ-6 Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-benzoyloxyglutaronitrile + H2O enantiomeric excess: 95%, 5 h Rhodococcus erythropolis (S)-3-benzoyloxy-4-cyanobutyramide
-
 ?
3-benzoyloxyglutaronitrile + H2O enantiomeric excess: 95%, 5 h Rhodococcus erythropolis AJ270 (S)-3-benzoyloxy-4-cyanobutyramide
-
 ?
3-benzyloxyglutaronitrile + H2O enantiomeric excess: 69%, 30 min Rhodococcus erythropolis 3-benzyloxy-4-cyanobutyramide
-
 ?
3-benzyloxyglutaronitrile + H2O enantiomeric excess: 69%, 30 min Rhodococcus erythropolis AJ270 3-benzyloxy-4-cyanobutyramide
-
 ?

Synonyms

Synonyms Comment Organism
NHase
-
 Rhodococcus erythropolis
nitrile hydratase
-
 Rhodococcus erythropolis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 YHJ3 Rhodococcus erythropolis
25
-
 YHJ1 Rhodococcus erythropolis