Any feedback?
Literature summary for 4.2.1.84 extracted from
- High resolution X-ray molecular structure of the nitrile hydratase from Rhodococcus erythropolis AJ270 reveals posttranslational oxidation of two cysteines into sulfinic acids and a novel biocatalytic nitrile hydration mechanism (2007), Biochem. Biophys. Res. Commun., 362, 319-324.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop method, X-ray diffraction, resolution: 1.3 A, ferric ion coordinating residues | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | - |
- |
- |
| Rhodococcus erythropolis AJ270 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | alphaCys112 and alphaCys114 are modified to cysteine sulfinic acids and isobutyronitrile is included in active site, evidence from crystallographic data | Rhodococcus erythropolis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| sonication, (NH4)2SO4 fractionation, DEAE-Sephacel/phenyl Sepharose/Sephacryl S200 column chromatography | Rhodococcus erythropolis |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | alphabeta | Rhodococcus erythropolis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NHase | - |
Rhodococcus erythropolis |
| nitrile hydratase | - |
Rhodococcus erythropolis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
