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Literature summary for 4.2.1.84 extracted from

  • Kashiwagi, M.; Fuhshuku, K.I.; Sugai, T.
    Control of the nitrile-hydrolyzing enzyme activity in Rhodococcus rhodochrous IFO 15564: preferential action of nitrile hydratase and amidase depending on the reaction condition factors and its application to the one-pot preparation of amides from aldehydes (2004), J. Mol. Catal. B, 29, 249-258.
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Search for more literature for 4.2.1.84

Application

Application Comment Organism
synthesis production of 2-naphthylacetamide by one-pot chemo-enzymatic conversion Rhodococcus rhodochrous

Organic Solvent Stability

Organic Solvent Comment Organism
dimethylformamide 30% v/v, no residual activity Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
-
-
Rhodococcus rhodochrous IFO 15564
-
-
-

Storage Stability

Storage Stability Organism
-20°C, 2 weeks, 90% residual activity, with addition of 8% glycerol, complete loss of activity within one week Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthylnitrile + H2O
-
 Rhodococcus rhodochrous 1-naphthylamide
-
 ?
1-naphthylnitrile + H2O
-
 Rhodococcus rhodochrous IFO 15564 1-naphthylamide
-
 ?
2-furonitrile + H2O
-
 Rhodococcus rhodochrous 2-furoamide
-
 ?
2-furonitrile + H2O
-
 Rhodococcus rhodochrous IFO 15564 2-furoamide
-
 ?
2-naphthylacetonitrile + H2O
-
 Rhodococcus rhodochrous 2-naphthylacetamide
-
 ?
2-naphthylacetonitrile + H2O
-
 Rhodococcus rhodochrous IFO 15564 2-naphthylacetamide
-
 ?
5-hydroxymethyl-2-furonitrile + H2O
-
 Rhodococcus rhodochrous 5-hydroxymethyl-2-furamide
-
 ?
5-hydroxymethyl-2-furonitrile + H2O
-
 Rhodococcus rhodochrous IFO 15564 5-hydroxymethyl-2-furamide
-
 ?
benzonitrile + H2O
-
 Rhodococcus rhodochrous benzamide
-
 ?
benzonitrile + H2O
-
 Rhodococcus rhodochrous IFO 15564 benzamide
-
 ?
trans-cinnamonitrile + H2O
-
 Rhodococcus rhodochrous trans-cinnamide
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Rhodococcus rhodochrous

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.4
-
 Rhodococcus rhodochrous

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Rhodococcus rhodochrous