Crystallization (Comment) | Organism |
---|---|
- |
Rhodococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-aminotetrazole monohydrate | - |
Rhodococcus sp. | |
Ca2+ | - |
Rhodococcus sp. | |
Cu2+ | - |
Rhodococcus sp. | |
Hg2+ | - |
Rhodococcus sp. | |
iodoacetic acid | - |
Rhodococcus sp. | |
N-bromosuccinimide | - |
Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
Isobutyronitrile | pH 7, 25ºC | Rhodococcus sp. | |
12.5 | - |
acetonitrile | pH 7, 25ºC | Rhodococcus sp. | |
14.3 | - |
Propionitrile | pH 7, 25ºC | Rhodococcus sp. | |
16.6 | - |
acrylonitrile | pH 7, 25ºC | Rhodococcus sp. | |
33.3 | - |
Benzonitrile | pH 7, 25ºC | Rhodococcus sp. | |
57 | - |
pivalonitrile | pH 7, 25ºC | Rhodococcus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rhodococcus sp. | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7 | Rhodococcus sp. |
32000 | - |
x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7 | Rhodococcus sp. |
278000 | - |
gel filtration | Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
- |
- |
Rhodococcus sp. 7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chromatography on Sepharose Q, phenyl-Sepharose CL-4B and Sephadex G-200, strain 7 | Rhodococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rhodococcus sp. |
3.8 | 4.6 | with 0.5% ammonium sulfate or acetonitrile in the incubation medium | Rhodococcus sp. |
4.9 | - |
with 0.5% acetonitrile and 0.5% yeast extract | Rhodococcus sp. |
485 | - |
purified enzyme | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetonitrile + H2O | 79% of the activity with acrylonitrile | Rhodococcus sp. | acetamide | - |
? | |
acetonitrile + H2O | 79% of the activity with acrylonitrile | Rhodococcus sp. 7 | acetamide | - |
? | |
acrylonitrile + H2O | - |
Rhodococcus sp. | 2-propenoic acid amide | i.e. acrylic acid amide | ? | |
acrylonitrile + H2O | - |
Rhodococcus sp. 7 | 2-propenoic acid amide | i.e. acrylic acid amide | ? | |
benzonitrile + H2O | 19.4% of the activity with acrylonitrile | Rhodococcus sp. | benzoic acid amide | - |
? | |
isobutyronitrile + H2O | 62.5% of the activity with acrylonitrile | Rhodococcus sp. | isobutyric acid amide | - |
? | |
isobutyronitrile + H2O | 62.5% of the activity with acrylonitrile | Rhodococcus sp. 7 | isobutyric acid amide | - |
? | |
pivalonitrile + H2O | 62% of the activity with acrylonitrile | Rhodococcus sp. | 2,2-dimethylpropionic acid amide | - |
? | |
pivalonitrile + H2O | 62% of the activity with acrylonitrile | Rhodococcus sp. 7 | 2,2-dimethylpropionic acid amide | - |
? | |
propionitrile + H2O | 64% of the activity with acrylonitrile | Rhodococcus sp. | propionic acid amide | - |
? | |
propionitrile + H2O | 64% of the activity with acrylonitrile | Rhodococcus sp. 7 | propionic acid amide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, alpha + x * 32000, beta, SDS-PAGE, strain 7 | Rhodococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Rhodococcus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | about 60% of activity maximum at 30°C, about 50% of activity maximum at 60°C | Rhodococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
60 min, about 80% loss of activity | Rhodococcus sp. |
55 | - |
20 min, about 90% loss of activity | Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | about 60% of activity maximum at pH 5, about 55% of activity maximum at pH 9 | Rhodococcus sp. |