Any feedback?
Literature summary for 4.2.1.83 extracted from
- Enzymes responsible for degradation of 4-oxalmesaconic acid in Pseudomonas ochraceae (1983), J. Biochem., 93, 567-574.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | partially reversed by DTT | Pseudomonas straminea |  |
| PCMB | partially reversed by DTT | Pseudomonas straminea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
gel filtration | Pseudomonas straminea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas straminea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pseudomonas straminea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:phthalate-grown cell | - |
Pseudomonas straminea | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, enzyme gradually inactivated, complete loss of activity after 6 months | Pseudomonas straminea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E)-3-carboxy-5-oxo-2-hex-2-enedioic acid + H2O | - |
Pseudomonas straminea | 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate | i.e. 4-carboxy-4-hydroxy-2-oxoadipic acid, gamma-oxalcitramalate | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
assay at | Pseudomonas straminea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Pseudomonas straminea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 9.1 | 50% of activity maximum at pH 7.4 and 9.1 | Pseudomonas straminea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas straminea | focusing at 6°C | - |
5.1 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
